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Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

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Abstract

Tetraploids plants of Anthurium andraeanum “Arizona” were successfully induced after treating diploid tissue masses with colchicine. Masses originating from diploid aerial roots were treated with colchicine at three different concentrations (i.e., 0.1, 0.2, 0.3%) for about 3, 5 and 7 h, and then were transferred into Murashige and Skoog medium containing 3 mg/l BAP + 0.2 mg/l 2,4-D. After 60 days, the survival rate and numbers of regenerative shoots were scored. The high concentration and longer duration sharply reduced survival rate. In contrast, the regeneration of plantlets was not noticeably affected by colchicine. Tetraploid plants were obtained in all treatments, but the percentage of induced tetraploids ranged from 0.2 to 7.6%. The best induction was obtained with a 5-h, treatment with 0.3% colchicine. The stomatal size of tetraploid plants was larger than in diploid plants; however, the stomatal density was lower than in diploid plants. Tetraploid plants possessed stronger petioles, thicker and deeper green leaves, and thicker and longer lived spathes in comparison with diploid plants. Abnormal spathes, such as double spathes or those lacking pedicels, were observed in tetraploid plants. Tetraploid plantlets could be regenerated via aerial roots; this technique could be applied to tetraploid plant propagation.

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Abbreviations

BAP:

6-Benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-Naphtaleneacetic acid

MS:

Murashige and Skoog (1962)

PI:

Propidium iodide

WPB:

Woody plant buffer

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Authors and Affiliations

  1. State Key Laboratory of Crop and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China

    Chao Chen & Xilin Hou

  2. Department of Life Science, Tangshan Normal College, Tangshan, China

    Chao Chen, Hongxin Zhang, Guilan Wang & Limin Tian

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  1. Chao Chen
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  2. Xilin Hou
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  3. Hongxin Zhang
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  4. Guilan Wang
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  5. Limin Tian
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Correspondence to Xilin Hou.

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Chen, C., Hou, X., Zhang, H. et al. Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro. Euphytica 181, 137–145 (2011). https://doi.org/10.1007/s10681-010-0344-3

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