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V3 protein encoded by mulberry crinkle leaf virus acts as a pathogenicity determinant in Nicotiana benthamiana

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Abstract

A potential pathogenicity determinant and its critical amino acid (aa) sequences of mulberry crinkle leaf virus (MCLV) were determined through expression of MCLV genes in Nicotiana benthamiana using a potato virus X (PVX) vector. Among six genes encoded by MCLV, expression of only V3 resulted in a severe downward-leaf-curling symptom in newly emerging leaves of N. benthamiana plants, which suggested that the V3 protein is a pathogenicity determinant of MCLV. Analyses of truncation mutations indicated that the 94 residues at the N terminus of V3 are sufficient to maintain the function of the pathogenicity determinant V3 in N. benthamiana.

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Acknowledgments

This work was supported by a grant from the Natural Science Foundation of Jiangsu Province, China (No. BK20151320), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 18KJA210001) and the Doctor Startup Fund Program of Jiangsu University of Science and Technology (No. 1732931601).

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Authors and Affiliations

  1. College of Biotechnology, Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, China

    Quan-You Lu, Jing Yu, Yong-Yuan Cheng, Xin Sun & Lei Yang

  2. Key Laboratory of Genetic Improvement of Silkworm and Mulberry, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212018, Jiangsu, China

    Quan-You Lu

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  1. Quan-You Lu
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  2. Jing Yu
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  3. Yong-Yuan Cheng
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  4. Xin Sun
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  5. Lei Yang
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Contributions

QYL conceived and designed the experiments. QYL, JY, YYC, and LY performed the experiments. XS cultivated the N. benthamiana plants. QYL wrote the paper. All of the authors read and approved the final manuscript.

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Correspondence to Quan-You Lu.

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Lu, QY., Yu, J., Cheng, YY. et al. V3 protein encoded by mulberry crinkle leaf virus acts as a pathogenicity determinant in Nicotiana benthamiana. Eur J Plant Pathol 157, 141–149 (2020). https://doi.org/10.1007/s10658-020-01991-1

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