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Molecular characterization and phylogenetic analysis of hop stunt viroid isolates from sweet cherry in China

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Abstract

Hop stunt viroid (HSVd), a member of the family Pospiviroidae, infects a large number of plants and causes substantial economic losses. In this study, HSVd was detected in dappled fruits of sweet cherry and then molecularly characterized. We sequenced 164 cDNA clones from six sweet cherry cultivars in the Shandong Province of China and identified 23 HSVd sequence variants, which ranged in size between 293 and 303 nucleotides (nt) and shared 97~99% sequence identity with each other. Characterization of HSVd variants revealed that three were predominant in sweet cherry trees. Phylogenetic analysis showed that most of the HSVd variants isolated from sweet cherry were clustered within the plum-type group and that two variants were clustered with the recombinant P-C group, regardless of geographic origin. This study identified 23 HSVd variants and provided the molecular characterization of viroid infection (HSVd) in sweet cherry in China.

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Acknowledgements

This research was supported by Research Funds for Youth of Shandong Academy of Agricultural Sciences (2016YQN25) and Special Found for Fruit Innovation Team of Shandong Modern Agricultural Technology System (SDAIT-06-04).

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Authors and Affiliations

  1. Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People’s Republic of China

    Li Xu, Jiawei Wang, Xin Chen, Dongzi Zhu, Hairong Wei & Qingzhong Liu

  2. US Department of Agriculture, ARS, Molecular Plant Pathology Laboratory, Room 214, Building 004 BARC West, Beltsville, MD, 20705, USA

    Rosemarie W. Hammond

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  1. Li Xu
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  2. Jiawei Wang
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  3. Xin Chen
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  4. Dongzi Zhu
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  5. Hairong Wei
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  6. Rosemarie W. Hammond
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  7. Qingzhong Liu
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Correspondence to Rosemarie W. Hammond or Qingzhong Liu.

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Xu, L., Wang, J., Chen, X. et al. Molecular characterization and phylogenetic analysis of hop stunt viroid isolates from sweet cherry in China. Eur J Plant Pathol 154, 705–713 (2019). https://doi.org/10.1007/s10658-019-01693-3

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  • DOI: https://doi.org/10.1007/s10658-019-01693-3

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