Abstract
Hop stunt viroid (HSVd) has been detected in many woody species including grapevine, citrus, peach, plum, apricot, and almond. Here, we report the first detection of HSVd in jujube trees (Ziziphus jujuba). Ninety-five samples were collected from jujube trees in Cangzhou, Baoding, and Handan of the Hebei province of China in June 2006. An additional 70 samples were collected from Taigu, the Shanxi province of China in August 2008. From these samples, low molecular weight RNAs were extracted for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), hybridisation, and reverse transcription polymerase chain reaction (RT-PCR) assays. HSVd was detected in three of the 165 samples, giving an infection rate of 1.8%. The sequences of the HSVd isolates from jujube trees shared 92.6–92.8% homology with the first reported HSVd sequence (GenBank: X00009). Using alignment and phylogenetic analyses, the HSVd sequence variants from jujube were classified in the plum subgroup and were found to be more conserved than other HSVd subgroups.
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Acknowledgements
This work was supported by grants from the National Basic Research and Development Programme of China (973 Programme) (No. 2009CB119200 and No. 2006 CB100203), the National Natural Science Foundation of China (No.30771403) , the National Key Technologies Research and Development Programme (No.2006 BAD08A14) , the National High Technology Research and Development Programme (863 Programme) (No.2006AA10Z432), the Beijing Natural Science Foundation of China (No. 6072022), and the Opening Project of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences. This work was also supported by NSFC-JSPS Joint Research Project between China and Japan (30811140157). We especially thank Prof. Teruo Sano at Hirosaki University of Japan for his valuable comments and the critical reading of this manuscript. We also thank Ms. Ailing Zhao at the Fruit Tree Research Institute of Shanxi Academy of Agricultural Sciences, for her kind help in collecting jujube tree samples.
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GenBank accession numbers
GenBank accession numbers for the sequence variants identified for HSVd isolated from Jujube are FJ771011-FJ771023.
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Zhang, B., Liu, G., Liu, C. et al. Characterisation of Hop stunt viroid (HSVd) isolates from jujube trees (Ziziphus jujuba) . Eur J Plant Pathol 125, 665–669 (2009). https://doi.org/10.1007/s10658-009-9504-5
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DOI: https://doi.org/10.1007/s10658-009-9504-5