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Transcriptome profiling helps to identify potential and true molecular switches of stealth to brute force behavior in Pectobacterium atrosepticum during systemic colonization of tobacco plants

  • SI: Plant Pathology for Innovative Agroecology
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Abstract

In the present study, we have monitored the process of systemic plant colonization by the plant pathogenic bacterium Pectobacterium atrosepticum (Pba) using RNA-Seq analysis in order to compare bacterial traits under in planta and in vitro conditions and to reveal potential players that participate in switching from stealth to brute force strategy of the pathogen. Two stages of tobacco plant colonization have been assayed: i) the initial one associated with visually symptomless spread of bacteria throughout the host body via primary xylem vessels where bacterial emboli were formed (stealth strategy), and ii) the advanced stage coupled with an extensive colonization of core parenchyma and manifestation of soft rot symptoms (brute force strategy). Plant-inducible genes in Pba and potential players switching the pathogen’s behavior were revealed. Genes from the cfa locus responsible for the production of coronafacic acid displayed the strongest induction in the asymptomatic zone relative to the symptomatic one and were shown experimentally to act as the true strategy “switchers” of Pba behavior in planta. Surprisingly, cfa genes appeared to be unnecessary for establishment of the asymptomatic stage of plant colonization but were required for the transition to soft-rot-associated symptomatic stage coupled with over-induction of jasmonate-mediated pathway in the plant.

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Acknowledgments

We would like to express our gratitude to Dr. Fabienne Guillon (INRA, France) for the INRA-RU2 antibody. This study was partially supported by the Russian Science Foundation (project No. 15-14-10022).

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Federal Research Center “Kazan Scientific Center of RAS”, Kazan, Russia

    Vladimir Gorshkov, Rim Gubaev, Olga Petrova, Amina Daminova, Natalia Gogoleva, Marina Ageeva, Olga Parfirova & Yuri Gogolev

  2. Kazan Federal University, Kazan, Russia

    Vladimir Gorshkov, Amina Daminova, Natalia Gogoleva, Olga Parfirova & Yuri Gogolev

  3. Department of Molecular Biology, Faculty of Biology, Belarusian State University, Minsk, Belarus

    Maxim Prokchorchik & Yevgeny Nikolaichik

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  1. Vladimir Gorshkov
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Fig. S1.

Transcript levels of P. atrosepticum operon located genes (according to DOOR2 database), which show different expression in asymptomatic zone 1 compared to in vitro. Zone 1 is designated in fig. 1. (GIF 34 kb)

High resolution image (TIF 90 kb)

Fig. S2.

Transcript levels of P. atrosepticum operon located genes (according to DOOR2 database), which show different expression in symptomatic zone 2 compared to in vitro. Zone 2 is designated in fig. 1. (GIF 39 kb)

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Gorshkov, V., Gubaev, R., Petrova, O. et al. Transcriptome profiling helps to identify potential and true molecular switches of stealth to brute force behavior in Pectobacterium atrosepticum during systemic colonization of tobacco plants. Eur J Plant Pathol 152, 957–976 (2018). https://doi.org/10.1007/s10658-018-1496-6

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