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Identification of pathotypes in Fusarium oxysporum f.sp. lentis

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Abstract

Fusarium oxysporum f.sp. lentis (Fol) is one of the most destructive diseases of lentil (Lens culinaris) worldwide. Although differences in in vitro growth pattern and aggressiveness have been observed in its populations, no pathotypes have been reported so far. The objective of this study was to check for the existence of differences in virulence in Fol and to develop a differential set of lentil accessions able to distinguish the virulence patterns. In a first step 28 lentil-resistant accessions reported elsewhere were inoculated with six isolates of Fol with different geographical origins, which showed a highly significant isolate × accession interaction. Based on their contrasting response, four accessions were selected as a putative differential set. In a second step we characterized the virulence pattern of 52 Fol isolates from Iran, Syria and Algeria using the differential set. This allowed the identification of seven different patterns of virulence, named as pathotypes 1 – 7. Pathotype 7 was virulent on all accessions studied. No correlation was found between the pathotype and the geographical origin of the isolates. This is the first report of the existence of pathotypes in Fol. Based on these results; lentil breeding strategies for resistance to the pathogen should be planned according to the prevalent pathotype present in the region.

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References

  • Armstrang, G. M., & Armstrang, J. K. (1974). Races of Fusarium oxysporum f.sp. pisi, causal agents of wilt of pea. Phytopathology, 64, 849–857.

    Article  Google Scholar 

  • Bayaa, B., Erskine, W., & Hamdi, A. (1995). Evaluation of a wild lentil collection for resistance to vascular wilt. Genetic Resources and Crop Evolution, 42, 231–235.

    Article  Google Scholar 

  • Bayaa, B., Erskine, W., & Singh, M. (1997). Screening lentil for resistance to Fusarium wilt: methodology and sources of resistance. Euphytica, 98, 69–74.

    Article  Google Scholar 

  • Bejiga, G., Abou-Zeid, N., Suliman, W., Ahmed, S., & Hassanein, A. (2001). Managing wilt and root rots of food legumes in the Nile Valley countries. Caravan, 15, 39–40.

    Google Scholar 

  • Belabid, L., & Fortas, Z. (2002). Virulence and vegetative compatibility of Algerian isolates of Fusarium oxysporum f. sp. lentis. Phytopathologia Mediterranea, 41, 179–187.

    Google Scholar 

  • Belabid, L., Baum, M., Fortas, Z., Bouznad, Z., & Eujayl, I. (2004). Pathogenic and genetic characterization of Algerian isolates of Fusarium oxysporum f.sp. lentis by RAPD and AFLP analysis. African Journal of Biotechnology, 3(1), 25–31.

    Article  CAS  Google Scholar 

  • Chaudhry, M. A., Ilyas, M. B., Ul-Hassan, I., & Ghazanfar, M. U. (2008). Sources of resistance from lentil international Fusarium wilt nursery 2006–7. Pakistan Journal of Phytopathology, 20(1), 122–124.

    Google Scholar 

  • Chen, W., Basandrai, A. K., Basandrai, D., Banniza, S., Bayaa, B., Buchwaldt, L., Davidson, J., Larsen, R., Rubiales, D., & Taylor, P. (2009). Lentil diseases and their management. In W. Erskine, F. J. Muehlbauer, A. Sarker, & B. Sharma (Eds.), The lentil: Botany, production and uses (pp. 262–281). Wallingford: CAB International.

    Chapter  Google Scholar 

  • Chen, W., Sharma, H. C., & Muehlbauer, F. J. (2011). Compendium of chickpea and lentil diseases and pests. St. Paul: The American Phytopathological Society.

    Google Scholar 

  • Datta, S., Choudhary, R. G., Shamin, M., Singh, R. K., & Dhar, V. (2011). Molecular diversity in Indian isolates of Fusarium oxysporum f.sp. lentis inciting wilt disease in lentil (Lens culinaris Medik). African Journal of Biotechnology, 10(38), 7314–7323.

    CAS  Google Scholar 

  • Di Pietro, A., Madrid, M. P., Caracuel, Z., Delgado-Jarana, J., & Roncero, M. I. G. (2003). Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Molecular Plant Pathology, 4, 315–325.

    Article  PubMed  Google Scholar 

  • El-Ashkar, F., Sarker, A., Haddad, N., Bayaa, B., El-Hassan, H., & Erskine, W. (2003). Registration of ‘Idlib-2’ Lentil. Crop Science, 43, 728–729.

    Article  Google Scholar 

  • El-Ashkar, F., Sarker, A., Erskine, W., Bayaa, B., El-Hassan, H., Kadah, N., & Karim, B. A. (2004a). Registration of ‘Idlib-3’ Lentil. Crop Science, 44, 2261–2262.

  • El-Ashkar, F., Sarker, A., Erskine, W., Bayaa, B., El-Hassan, H., Kadah, N., & Karim, B. A. (2004b). Registration of “Idlib-4” lentil. Crop Science, 44(6), 2261–2262.

  • Eujayl, I., Erskine, W., Bayaa, B., Baum, M., & Pehu, E. (1998). Fusarium vascular wilt in lentil: inheritance and identification of DNA markers for resistance. Plant Breeding, 117, 497–499.

    Article  Google Scholar 

  • Hamwieh, A., Udupa, S. M., Choumane, W., Sarker, A., Dreyer, F., Jung, C., & Baum, M. (2005). A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and localization of Fusarium vascular wilt resistance. Theoretical and Applied Genetics, 110, 669–677.

    Article  PubMed  CAS  Google Scholar 

  • Haware, M. P., & Nene, Y. L. (1982). Races of Fusarium oxysporum f.sp. ciceri. Plant Disease, 66, 809–810.

    Article  Google Scholar 

  • Hoagland, D.R., & Arnon, D.I. (1950). The water-culture method of growing plants without soil. California Agricultural Experiment Station Publications.

  • ICARDA. (2003). Ethiopia prepares to prevent recurrence of famine. Caravan, 18(19), 24–26.

    Google Scholar 

  • ICARDA. (2004). Annual report. Syria: ICARDA.

    Google Scholar 

  • Kamboj, R. K., Pandey, M. P., & Chaube, H. S. (1990). Inheritance of resistance to Fusarium wilt in Indian lentil germplasm (Lens culinaris Medik.). Euphytica, 50, 113–117.

    Article  Google Scholar 

  • Khare, M. N. (1980). Wilt of lentil. Technical Bulletin. Jabalpur, India: Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, M.P. India, 155pp.

  • Khare, M. N., Agrawal, S. C., Dhingra, O. D., & Kushwaha, L. S. (1975). Variability in the growth of eight strains of Fusarium oxysporum f.sp. lentis on different solid media. Indian Phytopathology, 28, 126–128.

    Google Scholar 

  • Kraft, J. M., Haware, M. P., Halila, H., & Bayaa, B. (2000). Soilborne diseases and their control. In R. Knight (Ed.), Linking research and marketing opportunities for pulses in 21st century (pp. 457–466). The Netherlands: Kluwer.

    Chapter  Google Scholar 

  • Machleb, H., Sarker, A., Kiwan, P., El-Hassan, H., & Erskine, W. (2007). Registration of “Hala” lentil. Journal of Plant Registration, 1, 40.

    Article  Google Scholar 

  • Michielse, C. B., & Rep, M. (2009). Pathogen profile update: Fusarium oxysporum. Molecular Plant Pathology, 10, 311–324.

    Article  PubMed  CAS  Google Scholar 

  • Mohammadi, N., Goltapeh, E. M., Babaie-Ahari, A., & Pouralibaba, H. (2011). Pathogenic and genetic characterization of Iranian isolates of Fusarium oxysporum f.sp. lentis by ISSR analysis. Journal of Agricultural Technology, 7(1), 63–72.

    Google Scholar 

  • Mohammdi, N., Pouralibaba, H. R., Mohammadi Goltapeh, A., Babaie Ahari, A., & Pakdaman Sararood, B. (2012). Advanced lentil lines screened for resistance to Fusarium oxysporum f.sp. lentis under greenhouse and field conditions. Phytoparasitica, 40, 69–76.

    Article  Google Scholar 

  • Nelson, P. E., Toussoun, T. A., & Marasas, W. F. O. (1983). Fusarium species. An illustrated manual for identification. University Park: The Pennsylvania State University Press.

    Google Scholar 

  • Omar, S. A., Salem, D. E., & Rizk, M. A. (1988). Sources of resistance to root-rot wilt disease complex of lentil. LENS Newsletter, 15(1), 37–39.

    Google Scholar 

  • Pandya, B. P., Pandey, M. P., & Singh, J. P. (1980). Development of pant-406 lentil, resistant to rust and wilt. LENS Newsletter, 7, 34–37.

    Google Scholar 

  • Pérez de la Vega, M., Fratini, R. M., & Muehlbauer, F. J. (2011). Lentil. In M. Pérez de la Vega, A. M. Torres, J. I. Cubero, & C. Kole (Eds.), Genetics, genomics and breeding of cool season grain legume (pp. 98–150). Enfield: Science Publishers, CRC-Press.

    Google Scholar 

  • Pouralibaba, H.R. (2010). Necessity of greenhouse evaluations in screening of lentil genotypes for resistance to fusarium wilt disease, caused by Fusarium oxysporum f.sp. lentis. (In Persian.) In Proc. 19th Iranian Plant Protection Congress, Tehran, Iran.

  • Pouralibaba, H. R., Sabaghpour, S. H., Mehrban, A., & Asghari, S. (2008). Selection of new lentil lines resistant to wilt disease, caused by Fusarium oxysporum f.sp. lentis, for Bilehsavar region, Moghan. (In Persian.). Seed and Plant Improvement Journal, 24(3), 429–444.

    Google Scholar 

  • Pouralibaba, H. R., Rubiales, D., & Fondevilla, S. (2015). Identification of resistance to Fusarium oxysporum f.sp. lentis in Spanish lentil germplasm. European Journal of Plant Pathology, 143(2), 399–405.

    Article  CAS  Google Scholar 

  • Sarker, A., Bayaa, B., & Erskine, W. (2001). Registration of six lentil germplasm lines with resistance to vascular wilt. Crop Science, 41, 1655.

    Article  Google Scholar 

  • Taheri, N., Falahati-Rastegar, M., Jafarpour, B., Bagheri, A. R., & Jahanbaghsh, V. (2010). Pathogenic and genetic characterization of Fusarium oxysporum f.sp. lentis by RAPD and IGS analysis in Khorasan Province. World Applied Science Journal, 9(3), 239–244.

    CAS  Google Scholar 

  • Taylor, P., Lindbeck, K., Chen, W., & Ford, R. (2007). Lentil diseases. In S. S. Yadav et al. (Eds.), Lentil: An ancient crop for modern times (pp. 291–313). The Netherlands: Springer.

    Chapter  Google Scholar 

  • Van der Plank, J. E. (1984). Disease resistance in plants (2nd ed.). New York: Academic.

    Google Scholar 

  • Woo, S. L., Zoina, A., Gel, S. G., Lorito, M., Nanni, B., Scala, F., & Noviello, C. (1996). Characterization of Fusarium oxysporum f. sp. phaseoli by pathogenic races, VCG, RFLPs, and RAPD. Phytopathology, 86, 966–973.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was carried out based on PhD educational mission No. 3972/200-28/1/1389 of first author from Agricultural Research, Education and Extension Organization (AREEO) of Iran and financed by project AGL2014-52871-R, co-financed by FEDER. SF was granted by a contract financed by the Ramón & Cajal program. The authors wish to thanks A. Moral, CSIC, Spain for technical assistance and Professor B. Bayaa, University of Aleppo, Syria, for helping to obtain Fol Algerian isolates.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain

    Hamid R. Pouralibaba, Diego Rubiales & Sara Fondevilla

  2. Dryland Agriculture Research Institute, 119, Maragheh, Iran

    Hamid R. Pouralibaba

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  1. Hamid R. Pouralibaba
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  2. Diego Rubiales
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  3. Sara Fondevilla
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Correspondence to Hamid R. Pouralibaba.

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Pouralibaba, H.R., Rubiales, D. & Fondevilla, S. Identification of pathotypes in Fusarium oxysporum f.sp. lentis . Eur J Plant Pathol 144, 539–549 (2016). https://doi.org/10.1007/s10658-015-0793-6

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