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Identification of resistance to Fusarium oxysporum f.sp. lentis in Spanish lentil germplasm

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Abstract

Vascular wilt caused by Fusarium oxysporum f.sp. lentis is a major disease of lentil (Lens culinaris) worldwide. In this study, 196 lentil landraces were screened for resistance under controlled and field conditions. In order to characterize different levels of incomplete resistance, a detailed method was developed to evaluate resistance to this pathogen under controlled conditions assessing symptoms in each leaf separately at weekly intervals and calculating Area under Disease Progress Curve (AUDPC). Consequently, selected accessions were screened in the field and the resistance confirmed. Twelve accession having good levels of resistance were identified. This article provides a new detailed method to evaluate incomplete resistance to the pathogen in lentil and identify new sources of resistance to the disease.

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Acknowledgments

This research was carried out based on PhD educational mission No. 3972/200-28/1/1389 of first author from Agricultural Research, Education and Extension Organization (AREEO) of Iran and by project AGL2011-22534 co-financed by FEDER. SF was granted by a contract financed by the Ramón & Cajal program. The authors are grateful to A. Moral, CSIC, Spain and A. Mehrban, Agriculture and Natural Resources Research Center of Moghan, Iran for helps and technical assistance.

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Authors and Affiliations

  1. Dryland Agriculture Research Institute, 119, Maragheh, Iran

    H. R. Pouralibaba

  2. Institute for Sustainable Agriculture, CSIC, Apdo. 4084, 14080, Córdoba, Spain

    D. Rubiales & S. Fondevilla

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  1. H. R. Pouralibaba
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  2. D. Rubiales
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  3. S. Fondevilla
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Correspondence to H. R. Pouralibaba.

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Pouralibaba, H.R., Rubiales, D. & Fondevilla, S. Identification of resistance to Fusarium oxysporum f.sp. lentis in Spanish lentil germplasm. Eur J Plant Pathol 143, 399–405 (2015). https://doi.org/10.1007/s10658-015-0692-x

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  • DOI: https://doi.org/10.1007/s10658-015-0692-x

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