Abstract
Greenhouse experiments were conducted to investigate the importance of tomato seeds as a route of pospiviroid dissemination. More than 7000 seeds were collected from tomato plants mechanically inoculated with Tomato apical stunt viroid, Citrus exocortis viroid, Columnea latent viroid and Potato spindle tuber viroid. Fruits and part of the seeds were found to be 100 % infected by the inoculated pospiviroid by means of RT-PCR testing. Much of the rest of the seeds were sown and produced about 4700 seedlings. None of the seedlings were found positive for the respective pospiviroid. This shows that pospiviroid seed transmission in tomato is very rare.
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Acknowledgments
The authors like to thank Roza Bojakova, Alina Vaitoniene, Andrius Stabingis, Daniel Goderniaux, Michel Facq, Elisabeth Demonty, Frédéric Fauche and Davide Luison for their indispensable help in the labs and in the greenhouses. This work was supported by the respective National Institutions in the frame of Project EUPHRESCO ‘DEP2’.
Compliance with Ethical Standards
The activity reported in this paper was financed by the respective National Institutions in the frame of Project EUPHRESCO “Detection and Epidemiology of Pospiviroids (DEP2)”. Specifically, DEP2 Project has been financed with a non-competitive funding mechanism. Each funder only pays for the participation of their own national researchers. The Authors did not have financial and non-financial conflicts of interest in the research. Moreover the research did not involve human participants and/or animals .
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Faggioli, F., Luigi, M., Sveikauskas, V. et al. An assessment of the transmission rate of four pospiviroid species through tomato seeds. Eur J Plant Pathol 143, 613–617 (2015). https://doi.org/10.1007/s10658-015-0707-7
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DOI: https://doi.org/10.1007/s10658-015-0707-7