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Distribution of tomato chlorotic dwarf viroid in floral organs of tomato

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Abstract

In situ hybridization was used to analyze the distribution pattern of Tomato chlorotic dwarf viroid (TCDVd) in floral organs of tomato plants. Following TCDVd invasion of floral organs, it became localized only in sepals at an early developmental stage, then reached other floral organs at the flower opening stage, with the exception of part of the placenta and ovules. When distribution of TCDVd was compared with that of Potato spindle tuber viroid (PSTVd), TCDVd was not detected in the outer integument around the embryo sac even though PSTVd was able to invade there, suggesting that such specific distribution might reflect the frequent occurrence of viroid disease on crops caused by PSTVd-seed transmission.

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Acknowledgements

We are grateful to T. Sano and S. Matsuura for effectual and useful comments to this study. We thank Y. Matsumura and S. Nagai for the maintenance of the plants. This study was supported, in part, by a Grant-in-Aid from The Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries, administered by the Ministry of Agriculture, Forestry and Fisheries in Japan.

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Authors and Affiliations

  1. National Institute of Floricultural Science, 2–1, Fujimoto, Tsukuba, 305–8519, Japan

    Yosuke Matsushita

  2. National Agricultural Research Center, Tsukuba, Ibaraki, 305–8666, Japan

    Tomio Usugi & Shinya Tsuda

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  1. Yosuke Matsushita
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  2. Tomio Usugi
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  3. Shinya Tsuda
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Correspondence to Shinya Tsuda.

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Matsushita, Y., Usugi, T. & Tsuda, S. Distribution of tomato chlorotic dwarf viroid in floral organs of tomato. Eur J Plant Pathol 130, 441–447 (2011). https://doi.org/10.1007/s10658-011-9766-6

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