Skip to main content

Advertisement

SpringerLink
Log in

Seed transmission of Pepino mosaic virus in tomato

  • Original Research
  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment without disinfection. Infection assays using indicator plants confirmed the presence of viable virus on the seeds. Seeds were distributed to ten different laboratories in three separate batches, where they were germinated and the young plants tested by ELISA. In total over 87,000 plants were tested and 23 positives detected, indicating an overall transmission rate of 0.026%. However, the observed seed transmission rates varied from 0.005% to 0.057%, depending on the seed batch used. Results clearly showed that PepMV can be transmitted from seeds contaminated with virus to seedlings, highlighting the risk of using seeds from PepMV-infected plants and the potential for seed transmission to contribute to the further spread of PepMV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aguilar, J. M., Hernandez-Gallardo, M. D., Cenis, J. L., Lacasa, A., & Aranda, M. A. (2002). Complete sequence of the Pepino mosaic virus RNA genome. Archives of Virology, 147, 2009–2015.

    Article  CAS  PubMed  Google Scholar 

  • Ali, A., Li, H., Schneider, W. L., Sherman, D. J., Gray, S., Smith, D., et al. (2006). Analysis of genetic bottlenecks during horizontal transmission of Cucumber mosaic virus. Journal of Virology, 80, 8345–8350.

    Article  CAS  PubMed  Google Scholar 

  • Betancourt, M., Fereres, A., Fraile, A., & Garcia-Arenal, F. (2008). Estimation of the effective number of founders that initiate an infection after aphid transmission of a multipartite plant virus. Journal of Virology, 82, 12416–12421.

    Article  CAS  PubMed  Google Scholar 

  • Blaszczak, V. (1964). Transmission of virus diseases by seed. Postepy Nauk Rolniczych, 86(2), 41–56.

    Google Scholar 

  • Córdoba-Sellés, M. C., García-Rández, A., Alfaro-Fernández, A., & Jordá-Gutiérrez, C. (2007). Seed transmission of Pepino mosaic virus and efficacy of tomato seed disinfection treatments. Plant Disease, 91, 1250–1254.

    Article  Google Scholar 

  • Cotillon, A. C., Girard, M., & Ducouret, S. (2002). Complete nucleotide sequence of the genomic RNA of a French isolate of Pepino mosaic virus (PepMV). Archives of Virology, 147, 2231–2238.

    Article  CAS  PubMed  Google Scholar 

  • Davino, S., Davino, M., Bellardi, M. G., & Agosteo, G. E. (2008). Pepino mosaic virus and Tomato chlorosis virus causing mixed infection in protected tomato crops in Sicily. Phytopathologia Mediterranea, 47, 35–41.

    CAS  Google Scholar 

  • French, C. J., Bouthillier, M., Bernardy, M., Ferguson, G., Sabourin, M., Johnson, R. C., et al. (2001). First report of Pepino mosaic virus in Canada and the United States. Plant Disease, 85, 1121.

    Article  Google Scholar 

  • Gibbs, A. J., & Gower, J. C. (1960). The use of a multi-transfer method in plant virus transmission studies: some statistical points arising in the analysis of results. Annals of Applied Biology, 48, 75–83.

    Article  Google Scholar 

  • Hanssen, I. M., Paeleman, A., Wittemans, L., Goen, K., Lievens, B., Bragard, C., et al. (2008). Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination. European Journal of Plant Pathology, 121, 131–46.

    Article  Google Scholar 

  • Hanssen, I. M., Paeleman, A., Vandewoestijne, E., Van Bergen, L., Bragard, C., Lievens, B., et al. (2009). Pepino mosaic virus isolates and differential symptomatology in tomato. Plant Pathology, 58, 450–460.

    Article  CAS  Google Scholar 

  • Hasiów, B., Borodynko, N., & Pospieszny, H. (2008). Complete genomic RNA sequence of the Polish Pepino mosaic virus isolate belonging to the US2 strain. Virus Genes, 36, 209–214.

    Article  PubMed  Google Scholar 

  • Jones, R. A. C., Koenig, R., & Lesemann, D. E. (1980). Pepino mosaic virus, a new potexvirus from pepino (Solanum muricatum). Annals of Applied Biology, 94, 61–68.

    Article  CAS  Google Scholar 

  • Krinkels, M. (2001). PepMV causes sticky problem. Prophyta, May 2001, 30–33.

  • Ling, K. (2007). Molecular characterization of two Pepino mosaic virus variants from imported tomato seed reveals high levels of sequence identity between Chilean and US isolates. Virus Genes, 34, 1–8.

    Article  CAS  PubMed  Google Scholar 

  • Ling, K.-S. (2008). Pepino mosaic virus on tomato seed: virus location and mechanical transmission. Plant Disease, 92, 1701–1705.

    Article  Google Scholar 

  • Ling, K.-S., Wintermantel, W. M., & Bledsoe, M. (2008). Genetic composition of Pepino mosaic virus population in North American greenhouse tomatoes. Plant Disease, 92, 1683–1688.

    Article  Google Scholar 

  • Lopez, C., Soler, S., & Nuez, F. (2005). Comparison of the complete sequences of three different isolates of Pepino mosaic virus: size variability of the TGBp3 protein between tomato and L. peruvianum isolates. Archives of Virology, 150, 619–627.

    Article  CAS  PubMed  Google Scholar 

  • Maroon-Lango, C. J., Guaragna, M. A., Jordan, R. L., Hammond, J., Bandla, M., & Marquardt, S. K. (2005). Two unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (EU like) US isolate. Archives of Virology, 150, 1187–1201.

    Article  CAS  PubMed  Google Scholar 

  • Moury, B., Fabre, F., & Senoussi, R. (2007). Estimation of the number of virus particles transmitted by an insect vector. Proceedings of the National Academy of Sciences of the United States of America, 104, 17891–17896.

    Article  CAS  PubMed  Google Scholar 

  • Mumford, R. A., & Metcalfe, E. J. (2001). The partial sequencing of the genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and Peru. Archives of Virology, 146, 2455–2460.

    Article  CAS  PubMed  Google Scholar 

  • Pagan, I., Cordoba-Selles, M. C., Martinez-Priego, L., Fraile, A., Malpica, J. M., Jorda, C., et al. (2006). Genetic structure of the population of Pepino mosaic virus infecting tomato crops in Spain. Phytopathology, 96, 274–279.

    Article  CAS  PubMed  Google Scholar 

  • Roggero, P., Masenga, V., Lenzi, R., Coghe, F., Ena, S., & Winter, S. (2001). First report of Pepino mosaic virus in tomato in Italy. Plant Pathology, 50, 798.

    Article  Google Scholar 

  • Salomone, A., & Roggero, P. (2002). Host range, seed transmission and detection by ELISA and lateral flow of an Italian isolate of Pepino mosaic virus. Journal of Plant Pathology, 84, 65–68.

    Google Scholar 

  • Soler, S., Cebolla-Cornejo, J., Prohens, J., & Nuez, F. (2000). El Pepino Mosaic Virus (PepMV), una nueva amenaza para el cultivo del tomate. II. Vida Rural, 119, 48–52.

    Google Scholar 

  • Spence, N. J., Basham, J., Mumford, R. A., Hayman, G., Edmondson, R., & Jones, D. R. (2006). Effect of Pepino mosaic virus on the yield and quality of glasshouse-grown tomatoes in the UK. Plant Pathology, 55, 595–606.

    Article  Google Scholar 

  • Van der Vlugt, R. A. A., Stijger, C. C. M. M., & Verhoeven, J. T. J. (2000). First report of Pepino mosaic virus on tomato. Plant Disease, 84, 103.

    Article  Google Scholar 

  • Verhoeven, J. T. J., van der Vlugt, R., & Roenhorst, J. W. (2003). High similarity between tomato isolates of Pepino mosaic virus suggests a common origin. European Journal of Plant Pathology, 109, 419–425.

    Article  CAS  Google Scholar 

  • Yang, Y., Kim, K. S., & Anderson, E. J. (1997). Seed transmission of Cucumber mosaic virus in Spinach. Phytopathology, 87, 924–931.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was financed by the European Commission in the 6th Framework Programme (PEPEIRA EC contract No 044189). The authors thank dr. Bart Thomma for critically reading this manuscript, Syngenta Seeds for providing seed harvesting procedures and De Ruiter Seeds for supplying tomato seeds. Research Center Hoogstraten (Hoogstraten, Belgium) and Research Station for Vegetable Production (Sint-Katelijne-Waver, Belgium) are acknowledged for support in seed production and grow-out trials.

Author information

Authors and Affiliations

  1. Scientia Terrae Research Institute, Fortsesteenweg 30a, 2860, Sint-Katelijne-Waver, Belgium

    Inge M. Hanssen

  2. Central Science Laboratory, Sand Hutton, York, YO41 1 LZ, UK

    Rick Mumford & Jeff Peters

  3. Norwegian Institute of Agricultural and Environmental Research, Plant Health and Plant Protection Division, Høgskoleveien 7, 1432, Aas, Norway

    Dag-Ragnar Blystad

  4. Universidade de Trás-os Montes e Alto Douro, Plant Protection Department, PO Box 1013, 5000-801, Vila Real, Portugal

    Isabel Cortez & Ana-Maria Pereira

  5. Institute of Plant Protection—National Research Institute, Department of Virology and Bacteriology, Węgorka 20, 60-318, Poznan, Poland

    Beata Hasiów-Jaroszewska & Henryk Pospieszny

  6. Plant Protection Institute, Panaiot Volov Srt., 35, 2230, Kostinbrod, Bulgaria

    Dimitrinka Hristova

  7. Universidad Politécnica de Madrid, Departamento de Biotechnología, Avenida Complutense s/n, 28040, Madrid, Spain

    Israel Pagán

  8. National Institute of Biology, Vecna pot 111, 1000, Ljubljana, Slovenia

    Maja Ravnikar

  9. Wageningen University Greenhouse Horticulture, PO box 20, 2665, MV, Bleiswijk, The Netherlands

    Ineke Stijger

  10. Centro di Ricerca per la Patologia Vegetale, Via Carlo Guiseppe Bertero 22, 0016, Rome, Italy

    Laura Tomassoli

  11. Benaki Phytopathological Institute, Lab. Virology, 8S. Delta str., 145 61, Kifissia Athens, Greece

    Christina Varveri

  12. Plant Research International, P.O. Box 16, 6700, AA, Wageningen, the Netherlands

    René van der Vlugt

  13. Aarhus University, Faculty of Agricultural Sciences, Forsøgsvej 1, 4200, Slagelse, Denmark

    Steen Lykke Nielsen

Authors
  1. Inge M. Hanssen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rick Mumford
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dag-Ragnar Blystad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Isabel Cortez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Beata Hasiów-Jaroszewska
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dimitrinka Hristova
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Israel Pagán
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ana-Maria Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jeff Peters
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Henryk Pospieszny
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Maja Ravnikar
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Ineke Stijger
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Laura Tomassoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Christina Varveri
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. René van der Vlugt
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Steen Lykke Nielsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Inge M. Hanssen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hanssen, I.M., Mumford, R., Blystad, DR. et al. Seed transmission of Pepino mosaic virus in tomato. Eur J Plant Pathol 126, 145–152 (2010). https://doi.org/10.1007/s10658-009-9528-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-009-9528-x

Keywords

Search

Navigation