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Identification of pathovars and races of Pseudomonas syringae, the main causal agent of bacterial disease in pea in North-Central Spain, and the search for disease resistance

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Abstract

A total of 298 bacterial isolates were collected from pea cultivars, landraces and breeding lines in North-Central Spain over several years. On the basis of biochemical-physiological characteristics and molecular markers, 225 of the isolates were identified as Pseudomonas syringae, either pv. pisi (110 isolates) or pv. syringae (112), indicating that pv. syringae is as frequent as pv. pisi as causal agent of bacterial diseases in pea. Most strains (222) were pathogenic on pea. Further race analyses of P. syringae pv. pisi strains identified race 4 (59.1% of the isolates of this pathovar), race 2 (20.0%), race 6 (11.8%), race 5 (3.6%) and race 3 (0.9%). Five isolates (4.6%) showed a not-previously described response pattern on tester pea genotypes, which suggests that an additional race 8 could be present in P. syringae pv. pisi. All the isolates of P. syringae pv. syringae were highly pathogenic when inoculated in the tester pea genotypes, and no significant pathogenic differences were observed. Simultaneous infections with P. syringae pv. pisi and pv. syringae in the same fields were observed, suggesting the importance of resistance to both pathovars in future commercial cultivars. The search for resistance among pea genotypes suitable for production in this part of Spain or as breeding material identified the presence of resistance genes for all P. syringae pv. pisi races except for race 6. The pea cultivars Kelvendon Wonder, Cherokee, Isard, Iceberg, Messire and Attika were found suitable sources of resistance to P. syringae pv. syringae.

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Abbreviations

Ppi :

Pseudomonas syringae pv. pisi, according to Vivian and Mansfield (1993)

Psy :

Pseudomonas syringae pv. syringae, according to Vivian and Mansfield (1993)

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Acknowledgements

This research was supported by the Junta de Castilla y León ITACYL 2004/845 and INIA RTA 2006-00077-00-00 projects, and by an INIA personal Ph.D. grant to A Martin-Sanz. We wish to thank Dr. R. Samson (INRA, France) and Dr. J. Taylor (HRW-I, United Kingdom) for supplying the strain collections.

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Authors and Affiliations

  1. Consejería de Agricultura y Ganadería, Junta de Castilla y León, Instituto Tecnológico Agrario, Ctra Burgos km 119, 47071, Valladolid, Spain

    Alberto Martín-Sanz & Constantino Caminero

  2. Consejería de Agricultura y Ganadería, Junta de Castilla y León, Centro Regional de Diagnóstico, Apdo. 61, 37080, Salamanca, Spain

    José Luis Palomo

  3. Área de Genética, Departamento de Biología Molecular, Universidad de León, 24071, León, Spain

    Marcelino Pérez de la Vega

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  1. Alberto Martín-Sanz
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  2. José Luis Palomo
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  3. Marcelino Pérez de la Vega
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  4. Constantino Caminero
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Correspondence to Constantino Caminero.

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Martín-Sanz, A., Palomo, J.L., Pérez de la Vega, M. et al. Identification of pathovars and races of Pseudomonas syringae, the main causal agent of bacterial disease in pea in North-Central Spain, and the search for disease resistance. Eur J Plant Pathol 129, 57–69 (2011). https://doi.org/10.1007/s10658-010-9691-0

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