Fisetin rescues retinal functions by suppressing inflammatory response in a DBA/2J mouse model of glaucoma

Abstract

Purpose

Glaucoma is a common chronic neurodegenerative disease, which could lead to visual loss. In this study, we aimed to investigate whether fisetin, a natural flavone with anti-inflammatory and antioxidant properties, is able to alleviate glaucoma.

Methods

We employed a DBA/2J mouse model which was treated with or without fisetin. Pattern electroretinogram (P-ERG), visual evoked potentials (VEPs) and intraocular pressure (IOP) were evaluated. Quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) were used to measure the expression levels of TNF-α, IL-1β and IL-6. Western blotting was performed to assess the activation of nuclear factor kappa-B (NF-κB).

Results

We found that DBA/2J mice treated with fisetin (10-30 mg/kg) showed improved P-ERG and VEP amplitudes and reduced IOP compared to untreated DBA/2J mice. In addition, there were more survived retinal ganglion cells (RGCs) and less activated microglia in fisetin-treated DBA/2J mice than those in untreated mice. Furthermore, secreted protein levels and mRNA levels of TNF-α, IL-1β and IL-6 were significantly repressed by fisetin. The phosphorylated p65 level in the nucleus was dramatically reduced in fisetin-treated mice compared to it in untreated mice. Our results demonstrate that fisetin may exert its function through regulating cytokine productions and inhibiting NF-κB activation in the retina.

Conclusion

In conclusion, fisetin is able to promote the visual functions of DBA/2J mice by inhibiting NF-κB activation.

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Change history

  • 15 November 2019

    The Editor-in-Chief of Documenta Ophthalmologica is publishing an editorial expression of concern for the manuscript "Fisetin rescues retinal functions by suppressing inflammatory response in a DBA/2J mouse model of glaucoma” by Li et al. (2019) [1].

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Correspondence to Jiaxiang Shen.

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All procedures performed in studies involving animal were in accordance with the ethical standards of the institutional and/or national research committee. Animal studies were approved by the People’s Hospital of Rizhao, and all efforts were made to minimize the number of animals used and their suffering.

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Li, L., Qin, J., Fu, T. et al. Fisetin rescues retinal functions by suppressing inflammatory response in a DBA/2J mouse model of glaucoma. Doc Ophthalmol 138, 125–135 (2019). https://doi.org/10.1007/s10633-019-09676-9

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Keywords

  • Glaucoma
  • Fisetin
  • Retinal ganglion cells (RGCs)
  • Nuclear factor kappa-B (NF-κB)
  • DBA/2J mouse