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Protective effect of selenium on vincristine-induced peripheral neuropathy in PC12 cell line

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Abstract

Vincristine-induced peripheral neuropathy (VIPN) is the main side effect and major reason for neuropathic pain in cancer survivors treated with vincristine. Vincristine, a chemotherapeutic antimitotic drug, is used frequently in combination chemotherapy. The primary purpose of the current study was to assess the protective effect of sodium selenite (SSe) on VIPN in vitro. Cytotoxicity effects of vincristine were evaluated using PC12 cells as a neuronal model. The cell culture studies were conducted in three groups based on the various treatments, including vincristine, SSe, and co-exposure to both compositions. Cell viability and cell cycle analyses were performed using MTT assay and flow cytometry, respectively. The level of mRNA expression of Bax and Bcl-2 was determined using qRT-PCR. According to the results, vincristine decreased the survival rate of PC12 cells. After 24 and 48 h exposure to different concentrations of vincristine (0.1–20 μΜ), the survival rate of PC12 cells decreased as compared to the control group. The results showed that treatment with 5 μΜ of vincristine resulted in apoptosis of PC12 cells. Interestingly,co-incubation of these cells with SSe significantly reduced the cell damage induced by vincristine. Furthermore, vincristine induced the inhibition of the G2 phase in PC 12 cells, and using SSe in combination with vincristine eliminated the inhibition of the cell cycle in the G2 phase. Briefly, our in vitro preliminary study showed that SSe might protect PC12 cells from vincristine-induced peripheral neuropathy during chemotherapy.

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Data and analyses are available from the corresponding author and first authors on request.

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Acknowledgements

The authors acknowledge Iran University of Medical Sciences Research and Technology support.

Funding

This study was funded by Iran University of Medical Sciences.

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Author notes

  1. Davod Jafari and Seyed Sadegh Eslami have equally contributed.

Authors and Affiliations

  1. Student Research Committee, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Davod Jafari & Seyed Sadegh Eslami

  2. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Davod Jafari, Seyed Sadegh Eslami & Parastoo Tarighi

  3. Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Sara Malih

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  1. Davod Jafari
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  2. Seyed Sadegh Eslami
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Contributions

Conception and design of the study: DJ and SSE, Acquisition of data: SSE and DJ, Analysis and/or interpretation: SSE, DJ, SM, Drafting the manuscript: SM, DJ, SSE, Revising the manuscript critically for important intellectual content: PT, DJ and SM. Artwork preparation: DJ.

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Correspondence to Parastoo Tarighi.

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Jafari, D., Eslami, S.S., Malih, S. et al. Protective effect of selenium on vincristine-induced peripheral neuropathy in PC12 cell line. Cytotechnology 74, 539–547 (2022). https://doi.org/10.1007/s10616-022-00543-1

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  • DOI: https://doi.org/10.1007/s10616-022-00543-1

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