Abstract
MicroRNA (miRNA) is an endogenous regulatory small molecule RNA. Growing evidence shows that miRNA plays an important regulatory role in gene expression. Although miRNA is a more intensive regulatory noncoding RNA in recent years, few studies have investigated the regulation of targeting genes involved in bone repair. Meanwhile, as a negative bone regulator, previous studies showed that casein kinase 2-interacting protein 1 (CKIP-1) is closely associated with bone formation and regeneration. However, the gene knockout method may not be suitable for clinical application. Therefore, it was hypothesized that miRNA molecules can inhibit the expression of CKIP-1 and ultimately promote the osteogenesis process. The present study revealed that let-7i-5p plays an important role in the process of fracture healing by inhibiting the expression of CKIP-1. Related research provides a novel gene target for fracture healing.
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This work was supported by Grants from the National Nature Science Foundation of China (No.11432016, No.31370942).
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10616_2020_444_MOESM1_ESM.jpg
Supplementary material 1 (JPEG 2193 kb). Figure S1. Detection of cell surface markers and evaluation of multi-directional ability to identify BMSCs. (A) Cell surface markers CD29, CD44 and CD45 were detected by flow cytometry. (B) Detection of the cell surface marker CD90 (200X). (C) BMSCs stained with alkaline phosphatase following osteogenic induction at days 7, 14 and 21(200X). (D) BMSCs stained with oil red O following adipogenic induction at day 7 (200X). (E) BMSCs stained with Alcian blue following chondrogenic induction at day 14 (200X).
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Zhang, Y., Cheng, W., Han, B. et al. Let-7i-5p functions as a putative osteogenic differentiation promoter by targeting CKIP-1. Cytotechnology 73, 79–90 (2021). https://doi.org/10.1007/s10616-020-00444-1
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DOI: https://doi.org/10.1007/s10616-020-00444-1