Abstract
Adipose tissue derived mesenchymal stem cells (ADMSCs) may be an attractive therapeutic source for acute liver injury because of their high accessibility and non-invasiveness. Here, we investigated the therapeutic potentials of porcine ADMSCs for acute liver failure (ALF). The morphology, differentiation potential, expression patterns of cell surface markers and liver-specific genes were compared between the ADMSCs derived from the pigs with or without ALF. For therapeutic studies, the expanded porcine ADMSCs from either ALF pig (ALF-ADMSCs) or healthy control pig (Nor-ADMSCs) of passage 3 were transplanted into CCl4-induced ALF mice, and the liver histology and functional tests were performed at days 1, 7, 14, and 21 after cell transplantation. ALF-ADMSCs expressed higher mRNA level of hepatic growth factor (HGF) than the Nor-ADMSCs. Both ALF-ADMSCs and Nor-ADMSCs improved liver histology, functions, and mouse survival rate. Higher level of porcine hepatocyte-specific genes was seen in the livers of ALF-ADMSCs transplanted mice as compared to the Nor-ADMSCs transplanted mice. In particular, ALF-ADMSCs transplanted mice expressed significantly higher level of albumin and cytokeratin 18 in the liver tissues as compared to the Nor-ADMSCs transplanted mice. ALF-ADMSCs might be superior to Nor-ADMSCs in the treatment of ALF as the former possesses stronger hepatic differentiation potential.
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Acknowledgements
This work was supported by the Natural Science Foundation of Zhejiang Province (Grant ID: LY17H030013, to Pan Xiaoping), the Zhejiang Health Science Foundation (Grant ID: 2017KY114, to Pan Xiaoping), the Hangzhou Health Science Foundation (Grant ID: 2016Z06, to Liu Shourong), and the National Undergraduate Innovation Training Program (Grant ID: 201710344027, to Wang Tiantian).
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Liu, S., Guo, R., Hou, X. et al. Adipose-tissue derived porcine mesenchymal stem cells efficiently ameliorate CCl4-induced acute liver failure in mice. Cytotechnology 72, 327–341 (2020). https://doi.org/10.1007/s10616-020-00370-2
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DOI: https://doi.org/10.1007/s10616-020-00370-2