Abstract
Tumor growth and metastasis depend on vessel formation, and inhibition of angiogenesis of tumor by production of anti-angiogenic drugs should be a promising approach for cancer therapy. Tumstatin is an angiogenesis inhibitor. The anti-angiogenic activity of tumstatin is localized to the 54–132 amino acids. The gene fragment encoding amino acids 45–132 of tumstatin (tum-5) was subcloned into pcDNA3.1 (pcDNA-tum5). Tum-5 protein could be expressed and secreted in CHO cells after transfection. The conditioned medium (containing tum-5 protein) from the transfectant has an anti-angiogenic effect on HUVEC cells in vitro. The anti-tumor effect of pcDNA-tum5 on mice bearing S180 tumors was evaluated. The results showed that pcDNA-tum-5 has a significant inhibition activity in the growth of the tumors. This study suggests that the gene delivery of tum-5 may be an effective strategy for cancer therapy.
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This work has been supported by program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) in China.
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You, Y., Xue, X., Li, M. et al. Inhibition effect of pcDNA-tum-5 on the growth of S180 tumor. Cytotechnology 56, 97–104 (2008). https://doi.org/10.1007/s10616-007-9117-9
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DOI: https://doi.org/10.1007/s10616-007-9117-9