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Simplified Two-Step Purification of Hyaluronidase from Buthus martensii Manchurian Scorpion Venom

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Whole venom of Buthus martensii Karsch Manchurian scorpion was collected by electrostimulation and was fractionated by cation-exchange chromatography over Toyopearl CM650-M followed by ultrafiltration through an Amicon Ultra-15 membrane with a cut-off threshold of 10 kDa to isolate hyaluronidase. The specific activity of the purified hyaluronidase was 16,000 TRU/mg. The degree of purification calculated from the increase of specific activity was 71×; the yield, 75.6%. The electrophoretic and chromatographic purities were 88.4% and 82.5%, respectively.

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Acknowledgment

The work was supported by the Natural Science Foundation, Xinjiang Autonomous Region Youth Foundation (Grant No. 2021D01B105), and the National Natural Science Foundation, Chinese Youth Foundation (Grant No. 82104017).

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Authors and Affiliations

  1. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi, P. R. China

    A. Wali, Z. Yang, A. Arken, Y. Ali, Y. H. Wang, R. Kelaimu, A. Yili & H. A. Aisa

  2. University of Chinese Academy of Sciences, 100039, Beijing, P. R. China

    Z. Yang, A. Arken, Y. Ali, Y. H. Wang & R. Kelaimu

  3. A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, 100125, Tashkent, Uzbekistan

    G. T. Mavlonov

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  1. A. Wali
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  2. Z. Yang
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  3. A. Arken
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  4. Y. Ali
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  6. R. Kelaimu
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  7. G. T. Mavlonov
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  8. A. Yili
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  9. H. A. Aisa
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Corresponding author

Correspondence to A. Yili.

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Translated from Khimiya Prirodnykh Soedinenii, No. 2, March–April, 2023, pp. 299–301.

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Wali, A., Yang, Z., Arken, A. et al. Simplified Two-Step Purification of Hyaluronidase from Buthus martensii Manchurian Scorpion Venom. Chem Nat Compd 59, 354–356 (2023). https://doi.org/10.1007/s10600-023-03992-y

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  • DOI: https://doi.org/10.1007/s10600-023-03992-y

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