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Isolation and characterization of 11 microsatellite loci for the Sichuan snub-nosed monkey, Rhinopithecus roxellana

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Abstract

We report on the isolation and characterization of 11 microsatellite loci in the Sichuan snub-nosed monkey (Rhinopithecus roxellana) from AC-enriched genomic libraries. 20 microsatellite loci were screened from the libraries, of which 11 were polymorphic. The number of observed alleles among 32 samples of snub-nosed monkey ranged from three to nine. The observed and expected heterozygosity were 0.071–0.815 and 0.201–0.819, respectively. The high variability revealed in this study should be useful tools for further study on social structure and population genetics of snub-nosed monkey in the future.

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Acknowledgements

This project was funded by the key project of Natural Science Foundation of China (NSFC, No.30630016), NSFC project (No. 30570292) and CAS 100 Talent Programme. Thanks are given to Drs. X.J. Zhan, B.W. Zhang and L.F. Zhu for their valuable technical advice and laboratory assistance. Special thanks to Dr. Z.J. Zhang, Beijing Zoo and Beijing Endangered Animal Breeding Center (Daxing, Beijing) for providing Sichuan snub-nosed monkey samples.

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Correspondence to M. Li.

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Hao, Y.L., Liu, Z.J., Wu, H. et al. Isolation and characterization of 11 microsatellite loci for the Sichuan snub-nosed monkey, Rhinopithecus roxellana. Conserv Genet 8, 1021–1024 (2007). https://doi.org/10.1007/s10592-006-9234-6

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  • DOI: https://doi.org/10.1007/s10592-006-9234-6

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