Abstract
We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy–Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.
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References
MA Beaumont MW Bruford (1999) Microsatellites in conservation genetics DB Goldstein C Schlötterer (Eds) Microsatellite: Evolution and Application Oxford University Press New York 165–182
J Beheim K Eldegard G Bjørnstad M Isaksson G Sonerud O Heie H Klungland (2002) ArticleTitleDNA polymorphism in boreal owls (Aegolius funereus) J. Raptor Res. 36 218–219
MS Blouin (2003) ArticleTitleDNA-based methods for pedigree reconstruction and kinship analysis in natural populations Trends Ecol. Evol. 18 503–511 Occurrence Handle10.1016/S0169-5347(03)00225-8
DeSalle R, Schierwater B (eds.) (1998) Molecular Approaches to Ecology and Evolution. Birkhäuser Verlag, Basel
NJ Gemmell S Akiyama (1996) ArticleTitleAn efficient method for the extraction of DNA from vertebrate tissues Trends Genet. 12 338–339 Occurrence Handle10.1016/S0168-9525(96)80005-9 Occurrence Handle1:CAS:528:DyaK28Xls1yhtL0%3D Occurrence Handle8855658
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (Version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html
Y-C Hsu LL Severinghaus Y-S Lin S-H Li (2003) ArticleTitleIsolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis) Mol. Ecol. Notes 3 595–597 Occurrence Handle10.1046/j.1471-8286.2003.00523.x Occurrence Handle1:CAS:528:DC%2BD2cXks12ksg%3D%3D
M Isaksson H Tegelström (2002) ArticleTitleCharacterization of polymorphic microsatellite markers in a captive population of the eagle owl (Bubo bubo) used for supportive breeding Mol. Ecol. Notes 2 91–93 Occurrence Handle10.1046/j.1471-8286.2002.00156.x Occurrence Handle1:CAS:528:DC%2BD38Xls1ars7o%3D
C König F Weick J-H Becking (1999) Owls: A Guide to the Owls of the World Pica Press East Sussex
NM Korfanta NA Schable TC Glenn (2002) ArticleTitleIsolation and characterization of microsatellite DNA primers in burrowing owl (Athene cunicularia) Mol. Ecol. Notes 2 584–585 Occurrence Handle10.1046/j.1471-8286.2002.00326.x Occurrence Handle1:CAS:528:DC%2BD38XpvVSit74%3D
TC Marshall J Slate LEB Kruuk JM Pemberton (1998) ArticleTitleStatistical confidence for likelihood-based paternity inference in natural populations Mol. Ecol. 7 639–655 Occurrence Handle10.1046/j.1365-294x.1998.00374.x Occurrence Handle1:STN:280:DyaK1c3ps1Olsw%3D%3D Occurrence Handle9633105
JM Pemberton DW Coltman TN Coulson J Slate (1999) Using microsatellites to measure the fitness consequences of inbreeding and outbreeding DB Goldstein C Schlötterer (Eds) Microsatellite: Evolution and Application Oxford University Press New York 151–164
CR Primmer AP Møller H Ellegren (1996) ArticleTitleA wide range survey of cross-species microsatellite amplification in birds Mol. Ecol. 5 365–378 Occurrence Handle10.1046/j.1365-294X.1996.00092.x Occurrence Handle1:CAS:528:DyaK28XktlyntrY%3D Occurrence Handle8688957
G Seutin BN White P Boag (1991) ArticleTitlePreservation of avian blood and tissue samples for DNA analysis Can. J. Zool. 69 82–90 Occurrence Handle1:CAS:528:DyaK3MXlslylur4%3D
LL Severinghaus P Rothery (2001) ArticleTitleThe survival rate of Lanyu scops owls Otus elegans botelensis IBIS 143 540–546
AB Thode M Maltbie LA Hansen LD Green LJ Longmire (2002) ArticleTitleMicrosatellite markers for the Mexican spotted owl (Strix occidentalis lucida) Mol. Ecol. Notes 2 446–447 Occurrence Handle10.1046/j.1471-8286.2002.00267.x Occurrence Handle1:CAS:528:DC%2BD38XpvVSjtLs%3D
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Hsu, YC., Li, SH., Lin, YS. et al. Microsatellite Loci from Lanyu Scops Owl (Otus elegans botelensis) and their Cross-Species Application in Four Species of Strigidae. Conserv Genet 7, 161–165 (2006). https://doi.org/10.1007/s10592-005-5477-x
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DOI: https://doi.org/10.1007/s10592-005-5477-x