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Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers

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Abstract

Thirty-five new microsatellite loci from the sea cucumbers Holothurian scabra (Jaeger, 1833) and Apostichopus japonicas (Selenka, 1867) were screened and characterized using the method of magnetic bead enrichment. Of the twenty-four polymorphic loci tested, eighteen were consistent with Hardy-Weinberg equilibrium after a modified false discovery rate (B-Y FDR) correction, whereas six showed statistically significant deviations (CHS2 and CHS11: P<0.014 790; FCS1, FCS6, FCS8 and FCS14: P<0.015 377). Furthermore, four species of plesiomorphous and related sea cucumbers (Holothurian scabra, Holothuria leucospilota, Stichopus horrens and Apostichopus japonicas) were tested for mutual cross-amplification using a total of ninety microsatellite loci. Although transferability and universality of all loci were generally low, the results of the cross-species study showed that the markers can be applied to identify individuals to species according to the presence or absence of specific microsatellite alleles. The microsatellite markers reported here will contribute to the study of genetic diversity, assisted breeding, and population conservation in sea cucumbers, as well as allow for the identification of individuals to closely related species.

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Authors and Affiliations

  1. Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, 361021, China

    Jingbo Shangguan  (上官静波) & Zhongbao Li  (黎中宝)

  2. Fisheries College, Jimei University, Xiamen, 361021, China

    Jingbo Shangguan  (上官静波) & Zhongbao Li  (黎中宝)

Authors
  1. Jingbo Shangguan  (上官静波)
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  2. Zhongbao Li  (黎中宝)
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Correspondence to Zhongbao Li  (黎中宝).

Additional information

Supported by the Natural Science Foundation of Fujian Province (Nos. 2014J01133, 2017J01638), the National Natural Science Foundation of China (No. 31272668), and the Program for New Century Excellent Talents in Fujian Province University and the Foundation for Innovative Research Team of Jimei University, China (No. 2010A004)

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Shangguan, J., Li, Z. Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers. J. Ocean. Limnol. 36, 519–527 (2018). https://doi.org/10.1007/s00343-018-6315-6

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  • DOI: https://doi.org/10.1007/s00343-018-6315-6

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