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Aminoguanidine Changes Hippocampal Expression of Apoptosis-Related Genes, Improves Passive Avoidance Learning and Memory in Streptozotocin-Induced Diabetic Rats

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Abstract

Cognitive dysfunction occurs in patients with diabetes mellitus. The objective of this study was to examine whether bilateral intrahippocampal CA1 (intra-CA1) injection of aminoguanidine (AG) can either affect the Bcl-2 family gene expression or reduce the diabetic imposing abnormalities of passive avoidance learning (PAL) and memory. Rats were divided into five groups: control (C), control treated with normal saline (CS), control treated with AG (S-AG), diabetics (D), and diabetics treated with AG (D-AG). Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg). AG (30 μg/rat) or vehicle was administered intra-CA1 bilaterally at the onset of hyperglycemia. PAL was assessed 7 weeks later. Animals were killed, and hippocampus was dissected following the behavioral test. The expressions of Bax, Bcl-2, and Bcl-xl mRNAs were measured using semiquantitative RT-PCR technique. The result of passive avoidance task showed that AG significantly improved the cognitive performance in diabetic rats. Moreover, AG treatment decreased the levels of Bcl-2 and Bcl-xL expressions in diabetic group. The ratio of Bax/Bcl-2 and Bax/Bcl-xL decreased significantly in AG-treated diabetic animals. In conclusion, initial treatment with AG by intra-CA1 micro-injection improves the impaired passive avoidance task in STZ-induced diabetic rats which may be related to the decreased Bax/Bcl-2 and Bax/Bcl-xL ratios.

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  1. Babol University of Medical Sciences, Babol, Iran

    Maryam Arab Firouzjaei

  2. Zanjan University of Medical Sciences, Zanjan, Iran

    Mohammad Reza Jafari, Mehdi Eskandari, Iraj Jafari Anarkoli & Mohsen Alipour

  3. Neuroscience Research Group, Zanjan University of Medical Sciences, Zanjan, Iran

    Mohsen Alipour

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  1. Maryam Arab Firouzjaei
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Firouzjaei, M.A., Jafari, M.R., Eskandari, M. et al. Aminoguanidine Changes Hippocampal Expression of Apoptosis-Related Genes, Improves Passive Avoidance Learning and Memory in Streptozotocin-Induced Diabetic Rats. Cell Mol Neurobiol 34, 343–350 (2014). https://doi.org/10.1007/s10571-013-0018-5

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