Abstract
In this study, we investigated the osteogenic potential of a low cost, sustainable cellulosic scaffold made from decellularized water-melon rind (dWMR). The dWMR scaffold was later surface treated with polydopamine (PDA) to achieve a higher surface roughness and a protein adhesive, hydrophilic surface. The PDA coated dWMR scaffold was characterized through morphological, enzymatic activity and molecular analyses. The osteogenic differentiation of human mesenchymal stem cells (hMSCs) proved the osteoinductive potential of the dWMR scaffold. The PDA treatment supported the osteoinduction of hMSCs, as reflected in the raised expression level of the genes responsible for mineralization and formation of hydroxyapatite crystals compared to that for the dWMR.
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Acknowledgements
Authors thanks member of the Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran for their support in experiments and discussions.
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This research has partially support by grant in aid to AA from Pasteur Institute of Iran and from Shahid Beheshti University to HS.
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RB, MN: Writing and editing the manuscript, investigation, data acquisition and curation, formal analysis, visualization; SH: Investigation, data acquisition, formal analysis, data validation; AG: Formal analysis; MJ: Formal analysis; PYW and SH: Scientific support, formal analysis; AA and HS: Conceptualization, supervision, editing the manuscript, fund acquisition.
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Banaeyan, R., Nourany, M., Hosseini, S. et al. Polydopamine‐based surface functionalization of watermelon rind as a 3D nanofibrous cellulose scaffold for osteogenesis. Cellulose 31, 443–461 (2024). https://doi.org/10.1007/s10570-023-05611-z
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DOI: https://doi.org/10.1007/s10570-023-05611-z