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Novel strategy for obtaining uniformly dispersed silver nanoparticles on soluble cotton wound dressing through carboxymethylation and in-situ reduction: antimicrobial activity and histological assessment in animal model

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Abstract

A simple but cost-effective method for preparing a novel partially carboxymethylated cotton gauze (PCG) with silver nanoparticles (AgNPs; i.e., PCG/Ag) was developed through carboxymethylation modification and reduction of Ag nitrate using polyvinylpyrrolidone (PVP) as a coating and complexing agent. The Fourier transform infrared spectrum of PCG/Ag revealed an absorption band at 1610 cm−1 and no chemical conversion in the synthesis of AgNPs. The synthesis of spherical AgNPs with a diameter of 5–20 nm was confirmed by ultraviolet–visible spectroscopy and showed plasmon resonance peaks at 425 nm; X-ray diffraction analysis revealed peaks at 2θ = 38.3° and 44.2° and X-ray photoelectron spectroscopy showed two peaks at 374.2–376.9 and 368.2–371.0 eV. AgNPs attached to partly carboxymethylated cotton gauze were optimized by a one-factor-at-a-time approach; the optimized conditions for an antimicrobial effect with no cytotoxicity was 30 min of soaking in 0.4% (w/v) Ag nitrate and reduction in 0.5% (w/v) ascorbic acid in the presence of defined amounts of PVP with stirring. PCG/Ag-4 showed excellent water absorption exceeding 2000% of the initial value. The inhibition zone test and bacterial growth inhibition curves suggested that PCG/Ag had antimicrobial activity against Escherichia coli and Staphylococcus aureus. The cell viability and cell attachment assays confirmed that the PCG/Ag was not cytotoxic and promoted wound healing in an animal model. These results indicate that PCG/Ag is a safe and effective material for wound dressing. This novel in-situ reduction method for obtaining uniformly dispersed nanoparticles on a biomaterial for wound dressing can be applied on a large scale without sacrificing low cost and efficiency.

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References

  • Aymonier C, Schlotterbeck U, Antonietti L, Zacharias P, Thomann R, Tiller JC, Mecking S (2002) Hybrids of silver nanoparticles with amphiphilic hyperbranched macromolecules exhibiting antimicrobial properties. Chem Commun 8:3018–3019

    Article  CAS  Google Scholar 

  • Barkhordari S, Yadollahi M, Namazi H (2014) pH sensitive nanocomposite hydrogel beads based on carboxymethyl cellulose/layered double hydroxide as drug delivery systems. J Polym Res 21:454

    Article  CAS  Google Scholar 

  • Bera S, Gangopadhyay P, Nair KGM, Panigrahi BK, Narasimhan SV (2006) Electron spectroscopic analysis of silver nanoparticles in a soda-glass matrix. J Electron Spectrosc Relat Phenom 152:91–95

    Article  CAS  Google Scholar 

  • Chen J, Lan G, Li K, Liu S, Yu K, Liu J, Tang H, Dai F, Wu D (2016) Preparation of a partially carboxymethylated cotton gauze and study of its hemostatic properties. J Mech Behav Biomed Mater 62:407–416

    Article  CAS  PubMed  Google Scholar 

  • Donnadio A, Ambrogi V, Pietrella D, Pica M, Sorrentino G, Casciola M (2016) Carboxymethylcellulose films containing chlorhexidine–zirconium phosphate nanoparticles: antibiofilm activity and cytotoxicity. RSC Adv 6:46249–46257

    Article  CAS  Google Scholar 

  • Ellis LJA, Valsami-Jones E, Lead JR, Baalousha M (2016) Impact of surface coating and environmental conditions on the fate and transport of silver nanoparticles in the aquatic environment. Sci Total Environ 568:95–106

    Article  CAS  PubMed  Google Scholar 

  • Elsner JJ, Berdicevsky I, Zilberman M (2010) In vitro microbial inhibition and cellular response to novel biodegradable composite wound dressings with controlled release of antibiotics. Acta Biomater 7:325–336

    Article  CAS  PubMed  Google Scholar 

  • Escobedo-Lozano AY, Domard A, Velázquez CA, Goycoolea FM, Argüelles-Monal WM (2015) Physical properties and antibacterial activity of chitosan/acemannan mixed systems. Carbohydr Polym 115:707–714

    Article  CAS  PubMed  Google Scholar 

  • Eyler RW, Klug ED, Diephuis F (1947) Determination of degree of substitution of sodium carboxymethylcellulose. Anal Chem 19:24–27

    Article  CAS  Google Scholar 

  • Gang C, Jingdong Z, Munetaka O, Kazuyuki H (2006) Silver-nanoparticle-attached indium tin oxide surfaces fabricated by a seed-mediated growth approach. J Phys Chem Part B Condens Matter Mater Surf Interfaces Biophys 109:1204–1209

    Google Scholar 

  • Guo S, DiPietro LA (2010) Critical review in oral biology & medicine: factors affecting wound healing. J Dent Res 89:219–229

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guo A, Wang F, Lin W, Xu X, Tang T, Shen Y, Guo S (2014) Evaluation of antibacterial activity of N-phosphonium chitosan as a novel polymeric antibacterial agent. Int J Biol Macromol 67:163–171

    Article  CAS  PubMed  Google Scholar 

  • José VS (2010) Chronic wounds treated with iodinated cadexomer. Study of a series of clinical cases with Iodosorb. Revista de enfermeria? (Barcelona, Spain) 33:38–42

    Google Scholar 

  • Kučišec-Tepeš N (2016) The role of antiseptics and strategy of biofilm removal in chronic wound. Acta Med Croat 70:33–41

    Google Scholar 

  • Kumar V, Gundampati RK, Singh DK, Bano D, Jagannadham MV, Hasan SH (2016) Photoinduced green synthesis of silver nanoparticles with highly effective antibacterial and hydrogen peroxide sensing properties. J Photochem Photobiol B 162:374

    Article  CAS  PubMed  Google Scholar 

  • Kutsenko LI, Bochek AM, Vlasova EN, Volchek BZ (2005) Synthesis of carboxymethyl cellulose based on short fibers and lignified part of flax pedicels (boon). Russ J Appl Chem 78:2014–2018

    Article  CAS  Google Scholar 

  • Li X, Liu Y, Zhang J, You R, Qu J, Li M (2017) Functionalized silk fibroin dressing with topical bioactive insulin release for accelerated chronic wound healing. Mater Sci Eng C 72:394–404

    Article  CAS  Google Scholar 

  • Marin S, Vlasceanu GM, Tiplea RE, Bucur IR, Lemnaru M, Marin MM, Grumezescu AM (2015) Applications and toxicity of silver nanoparticles: a recent review. Curr Top Med Chem 15:1596–1604

    Article  CAS  PubMed  Google Scholar 

  • Nam JM, Park SJ, Mirkin CA (2002) Bio-barcodes based on oligonucleotide-modified nanoparticles. J Am Chem Soc 124:3820

    Article  CAS  PubMed  Google Scholar 

  • Nayak S, Kundu SC (2014) Sericin–carboxymethyl cellulose porous matrices as cellular wound dressing material. J Biomed Mater Res Part A 102(6):1928–1940

    Article  CAS  Google Scholar 

  • Petersen W, Rahmanian-Schwarz A, Werner J-O, Schiefer J, Rothenberger J, Hübner G, Schaller H-E, Held M (2016) The use of collagen based matrices in the treatment of full thickness wounds. Burns 42:1257–1264

    Article  PubMed  Google Scholar 

  • Pollini M, Paladini F, Sannino A, Maffezzoli A (2016) Development of hybrid cotton/hydrogel yarns with improved absorption properties for biomedical applications. Mater Sci Eng C 63:563–569

    Article  CAS  Google Scholar 

  • Pradeep KR, Abraham A (2016) PVP-coated naringenin nanoparticles for biomedical applications: in vivo toxicological evaluations. Chem Biol Interact 257:110–118

    Article  CAS  Google Scholar 

  • Schön G, Tummavuori J, Lindström B, Enzell CR, Swahn CG (1973) ESCA Studies of Ag, Ag2O and AgO. Acta Chem Scand 27:2623–2633

    Article  Google Scholar 

  • Shankar S, Rhim J-W (2017) Facile approach for large-scale production of metal and metal oxide nanoparticles and preparation of antibacterial cotton pads. Carbohydr Polym 163:137–145

    Article  CAS  PubMed  Google Scholar 

  • Siddiq AM, Parandhaman T, Begam AF, Das SK, Alam MS (2016) Effect of gemini surfactant (16-6-16) on the synthesis of silver nanoparticles: a facile approach for antibacterial application. Enzyme Microb Technol 95:118

    Article  CAS  PubMed  Google Scholar 

  • Sogol A, Sarvesh L, Song L (2016) Imparting commercial antimicrobial dressings with low-adherence to burn wounds. Burns J Int Soc Burn Inj 42:877–883

    Article  Google Scholar 

  • Song JE, Phenrat T, Marinakos S, Xiao Y, Liu J, Wiesner MR, Tilton RD, Lowry GV (2011) Hydrophobic interactions increase attachment of gum Arabic- and PVP-coated Ag nanoparticles to hydrophobic surfaces. Environ Sci Technol 45:5988–5995

    Article  CAS  PubMed  Google Scholar 

  • Song J, Zhang P, Cheng L, Liao Y, Xu B, Bao R, Wang W, Liu W (2015) Nano-silver in situ hybridized collagen scaffolds for regeneration of infected full-thickness burn skin. J Mater Chem B 3:4231–4241

    Article  CAS  Google Scholar 

  • Thuc DT, Huy TQ, Hoang LH, Tien BC, Chung PV, Thuy NT, Le A-T (2016) Green synthesis of colloidal silver nanoparticles through electrochemical method and their antibacterial activity. Mater Lett 181:173–177

    Article  CAS  Google Scholar 

  • Tomasz Z, Anna Z, Hanna K, Agnieszka M, Stefan T (2013) The evaluation of bacteria penetration by medical textiles for multiple use and disposable multilayer surgical drapes, according to the PN-EN ISO 22610 standard. Med Dosw Mikrob 64:261–270

    Google Scholar 

  • Wang Q, Liu S, Wang H, Yang Y (2016) In situ pore-forming alginate hydrogel beads loaded with in situ formed nano-silver and their catalytic activity. PCCP 18:12610–12615

    Article  CAS  PubMed  Google Scholar 

  • Wu S, Meng S (2005) Preparation of ultrafine silver powder using ascorbic acid as reducing agent and its application in MLCI. Mater Chem Phys 89:423–427

    Article  CAS  Google Scholar 

  • Ye D, Zhong Z, Xu H, Chang C, Yang Z, Wang Y, Ye Q, Zhang L (2016) Construction of cellulose/nanosilver sponge materials and their antibacterial activities for infected wounds healing. Cellulose 23:749–763

    Article  CAS  Google Scholar 

  • Zeng X, Mccarthy DT, Deletic A, Zhang X (2015) Silver/reduced graphene oxide hydrogel as novel bactericidal filter for point-of-use water disinfection. Adv Funct Mater 25:4344–4351

    Article  CAS  Google Scholar 

  • Zhao C-M, Wang W-X (2012) Importance of surface coatings and soluble silver in silver nanoparticles toxicity to Daphnia magna. Nanotoxicology 6:361–370

    Article  CAS  PubMed  Google Scholar 

  • Zhao Y, Xu Z, Lin T (2016) Barrier textiles for protection against microbes. In: Sun G (ed) Antimicrobial textiles. Woodhead Publishing, Cambridge, pp 225–245

    Chapter  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 51703185) and Fundamental Research Funds for Central Universities (Nos. XDJK2017B041 and XDJK2017C012).

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Author notes

  1. Tianyi Wu and Fei Lu have contributed equally to this work.

Authors and Affiliations

  1. College of Textile and Garments, Southwest University, Chongqing, 400715, China

    Tianyi Wu, Fei Lu, Qiuhan Wen, Kun Yu, Bitao Lu, Fangyin Dai & Guangqian Lan

  2. Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing, 400715, China

    Fei Lu, Fangyin Dai & Guangqian Lan

  3. The Ninth People’s Hospital of Chongqing, Chongqing, 400715, China

    Bao Rong

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  1. Tianyi Wu
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Correspondence to Guangqian Lan.

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Wu, T., Lu, F., Wen, Q. et al. Novel strategy for obtaining uniformly dispersed silver nanoparticles on soluble cotton wound dressing through carboxymethylation and in-situ reduction: antimicrobial activity and histological assessment in animal model. Cellulose 25, 5361–5376 (2018). https://doi.org/10.1007/s10570-018-1907-z

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