Abstract
To enhance the efficiency of 22-hydroxy-23,24-bisnorchol-4-ene-3-one (HBC) production at high substrate concentrations, the cyclodextrin-resting cell system was used for the bioconversion of phytosterols (PS) in this study. Two intermediates, identified as 4-ene-3-keto steroids and 27-hydroxy-4-ene-3-keto steroids, were accumulated at the early stage of the bioconversion and related to the oxygen bioavailability. We demonstrated that oxygen bioavailability was the main rate-limited factor at high substrate concentrations in the reaction system. Measures improving oxygen transfer rate in the reaction system significantly enhanced the bioconversion efficiency of PS to HBC with a space–time yield of 13.25 g/L/day.
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This work was supported by the National Natural Science Foundation of China (grant numbers 31570079, 21276083).
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Han, Y., Zhu, X., Wang, X. et al. Oxygen Bioavailability is a Rate-Limited Factor in Phytosterols Bioconversion Using a Cyclodextrin-Resting Cell System. Catal Lett 153, 1557–1563 (2023). https://doi.org/10.1007/s10562-022-04090-4
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DOI: https://doi.org/10.1007/s10562-022-04090-4