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Facile Fabrication of a Novel and Reusable 3D Laccase Reactor for Efficient Removal of Pollutants from Wastewater

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Abstract

Abstract

An ultra simple 3D bioreactor was prepared via Fe3+ linking to primary amines of melamine sponge (MeS) for cooperative complex formation with enzyme. Laccase worked as a model of enzyme and no chemical modification was employed during the material preparation. The 3D structure of MeS can enhance the enzyme loading amount up to 180 µg/mg, albeit MeS as monolithic material for enzyme immobilization is rather unexplored. The 3D bioreactor (MeS@Fe3+@laccase) also improved the catalytic efficiency and showed excellent reusability, stability, and reproducibility. In this study, the as-prepared bioreactor (10 mg) was successfully applied to the degradation of dyes and micropollutants, which has shown higher efficiency than free laccase in the large-scale decolorization of dyes and showed up to almost 100% removal of 2,4,6-trichlorophenol (2,4,6-TCP) and sulfisoxazole. Therefore, the MeS@Fe3+ with low cost, good stability and easy separation can be employed as a new enzyme carrier such as trypsin and lipase in biotechnological applications.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21575055).

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Authors and Affiliations

  1. State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China

    Yulin Zou, Fanpeng Ran, Qiangwei Huang, Xiaoyan Liu & Haixia Zhang

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  1. Yulin Zou
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  2. Fanpeng Ran
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  3. Qiangwei Huang
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  4. Xiaoyan Liu
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Correspondence to Haixia Zhang.

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Zou, Y., Ran, F., Huang, Q. et al. Facile Fabrication of a Novel and Reusable 3D Laccase Reactor for Efficient Removal of Pollutants from Wastewater. Catal Lett 149, 2706–2717 (2019). https://doi.org/10.1007/s10562-019-02732-8

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