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In vivo construction of tissue-engineered cartilage using adipose-derived stem cells and bioreactor technology

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Abstract

The present study aims to investigate the feasibility of tissue-engineered cartilage constructed in vivo and in vitro by dynamically culturing adipose-derived stem cells (ADSCs) with an articular cartilage acellular matrix in a bioreactor and subsequently implanting the cartilage in nude mice. ADSCs were proliferated, combined with three dimensional scaffolds (cell density: 5 × 107/mL) and subsequently placed in a bioreactor and culture plate for 3 weeks. In the in vivo study, complexes cultured for 1 week under dynamic or static states were subcutaneously implanted into nude mice and collected after 3 weeks. Indicators such as gross morphology, histochemistry and immunohistochemistry were examined. In the in vitro study, histological observation showed that most scaffolds in the dynamic group were absorbed, and cell proliferation and matrix secretion were significant. Positive staining of safranin-O and alcian blue II collagen stain in the dynamic group was significantly stronger than that in the static culture group. In the in vivo study, cartilage-like tissues formed in the specimens of the two groups. Histological examination showed that cell distribution in the dynamic group was relatively more uniform than in the static group, and matrix secretion was relatively stronger. Bioreactor culturing can promote ADSC proliferation and cartilage differentiation and is thus a suitable method for constructing tissue-engineered cartilage in vivo.

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Abbreviations

ADSCs:

Adipose-derived stem cells

PBS:

Phosphate-buffered saline

DMEM:

Dulbecco’s modified Eagle’s medium

FBS:

Foetal bovine serum

IACUC:

Institutional Animal Care and Use Committee

bFGF:

Basic fibroblast growth factor

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Acknowledgments

This study was funded by the National Science Foundation of China (30330570), Beijing Science and Technology Development Foundation (H060920050630), Major State Basic Science Research and Development Program of China (973, No. 2005CB5227074) and Medical Health Research Found Project of Chinese PLA (06Z057).

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Authors and Affiliations

  1. Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China

    Hongjun Kang, Shibi Lu, Jiang Peng, Shuyun Liu, Li Zhang, Jingxiang Huang, Xiang Sui, Bin Zhao, Aiyuan Wang, Wenjing Xu & Quanyi Guo

  2. Department of Spine Surgery, Tianjin Hospital, Tianjin, 300211, China

    Qiang Yang

  3. Critical Care Medicine, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China

    Qing Song

Authors
  1. Hongjun Kang
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  2. Shibi Lu
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  3. Jiang Peng
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  4. Qiang Yang
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  5. Shuyun Liu
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  6. Li Zhang
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  7. Jingxiang Huang
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  8. Xiang Sui
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  9. Bin Zhao
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  10. Aiyuan Wang
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  11. Wenjing Xu
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  12. Quanyi Guo
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  13. Qing Song
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Corresponding authors

Correspondence to Quanyi Guo or Qing Song.

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Kang, H., Lu, S., Peng, J. et al. In vivo construction of tissue-engineered cartilage using adipose-derived stem cells and bioreactor technology. Cell Tissue Bank 16, 123–133 (2015). https://doi.org/10.1007/s10561-014-9448-7

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  • DOI: https://doi.org/10.1007/s10561-014-9448-7

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