We have optimized the cultivation parameters for the microalgae Botryococcus braunii and Chlorella in a photobioreactor: duration of the cultivation cycle, aeration conditions, degree of enrichment of feed air with carbon dioxide, photoperiod and light/dark cycles. We have established that the greatest increase in biomass is observed when using the drain/replenish method, withdrawing 90% of the culture fluid in a 4-day cultivation cycle for Chlorella and a 7-day cycle for Botryococcus braunii, gradually increasing the carbon dioxide content in the feed air (from 0% in the first 24 hours to 2% toward the end of the cultivation cycle), 12 hours total for the light/dark cycle and a photoperiod of 9 hours.
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Translated from Khimiya i Tekhnologiya Topliv i Masel, No. 1, pp. 7 – 10, January – February, 2012.
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Kotelev, M.S., Novikov, A.A., Afonin, D.S. et al. Production of energy-dense biomass of microalgae Botryococcus braunii and Chlorella in a photobioreactor. Chem Technol Fuels Oils 48, 8–12 (2012). https://doi.org/10.1007/s10553-012-0328-1
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DOI: https://doi.org/10.1007/s10553-012-0328-1