Skip to main content
SpringerLink
Log in

Sustained release of insulin through skin by intradermal microdelivery system

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

An intradermal microdelivery system comprising a wafer having 150-μm long microneedles and an applicator was used for controlled release of insulin. Insulin was topically applied on microneedle-pretreated skin. In vitro percutaneous studies showed that the penetration amount of FITC-Insulin following microneedle pretreatment was enhanced significantly and continued to increase for several hours after the removal of donor phase. In vivo studies on diabetic rats confirmed that the pharmacodynamic profile of percutaneously administrated regular insulin was smooth, with the duration of action comparable to that of subcutaneously injected biphasic insulin. The effects of insulin concentration, duration and area of microneedle treatment on blood glucose lowering were studied in vivo. The results indicated that the duration of microneedle treatment and insulin concentration were key influencing factors to optimize blood glucose control. The extent of blood glucose lowering was in proportion to the area of microneedle treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

NBGL:

Normalized blood glucose level

AOC:

Area over the curve and below baseline

P-value:

Probability value

References

  • American Diabetes Association (ADA), Diabetes Care 25, S116 (2002)

    Article  Google Scholar 

  • A.H. Barnett, Diabet. Med. 20, 873–885 (2003)

    Article  Google Scholar 

  • H. Chen, H. Zhu, J. Zheng, D. Mou, J. Wan, J. Zhang, T. Shi, Y. Zhao, H. Xu, X. Yang, J. Control. Release (2009) doi: 10.1016/j.jconrel.2009.05.031

  • A. Davidson, B. Al-Qallaf, D.B. Das, Chem. Eng. Res. Des. 86, 1196–1206 (2008)

    Article  Google Scholar 

  • M.I. Haq, E. Smith, D.N. John, M. Kalavala, C. Edwards, A. Anstey, A. Morrissey, J.C. Birchall, Biomed. Microdevices 11, 35–47 (2009)

    Article  Google Scholar 

  • S. Henry, D.V. McAllister, M.G. Allen, M.R. Prausnitz, J. Pharm. Sci. 87, 922–925 (1998)

    Article  Google Scholar 

  • Y. Ito, E. Hagiwara, A. Saeki, N. Sugioka, K. Takada, Eur. J. Pharm. Sci. 29, 82–88 (2006)

    Article  Google Scholar 

  • Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council, Guide for the Care and Use of Laboratory Animals (National Academy, Washington, D.C., 1996)

    Google Scholar 

  • S. Kaushik, A.H. Hord, D.D. Denson, D.V. McAllister, S. Smitra, M.G. Allen, M.R. Prausnitz, Anesth. Analg. 92, 502–504 (2001)

    Article  Google Scholar 

  • E. Khafagy, M. Morishita, Y. Onuki, K. Takayamas, Adv. Drug Deliver. Rev. 59, 1521–1546 (2007)

    Article  Google Scholar 

  • X. Li, R. Zhao, Z. Qin, J. Zhang, S. Zhai, Y. Qiu, Y. Gao, B. Xu, S. H. Thomas, Am. J. Emerg. Med. (2008) doi: 10.1016/j.ajem.2008.10.001

  • W. Martanto, S.P. Davis, N.R. Holiday, J. Wang, H.S. Gill, M.R. Prausnitz, Pharm. Res. 21, 947–952 (2004)

    Article  Google Scholar 

  • W. Martanto, J.S. Moore, T. Couse, M.R. Prausnitz, J. Control, Release 112, 357–361 (2006)

    Article  Google Scholar 

  • D.V. McAllister, P.M. Wang, S.P. Davis, J. Park, P.J. Canatella, M.G. Allen, M.R. Prausnitz, PNAS 100, 13755–13760 (2003)

    Article  Google Scholar 

  • L. Nordquist, N. Roxhed, P. Griss, G. Stemme, Pharm. Res. 24, 1381–1388 (2007)

    Article  Google Scholar 

  • Organization for Economic Cooperation and Development (OECD), OECD Guidelines for Testing of Chemicals, No. 428: Skin Absorption: In vitro Method (OECD, 2004)

  • M.R. Prausnitz, Adv. Drug Deliver. Rev. 56, 581–587 (2004)

    Article  Google Scholar 

  • F.J. Verbaan, S.M. Bal, D.J. van den Berg, W.H.H. Groenink, H. Verpoorten, R. Lüttge, J.A. Bouwstra, J. Control, Release 117, 238–245 (2007)

    Article  Google Scholar 

  • P.M. Wang, M. Cornwell, J. Hill, M.R. Prausnitz, J. Invest. Dermatol. 126, 1080–1087 (2006)

    Article  Google Scholar 

  • J. Weng, Y. Li, W. Xu, L. Shi, Q. Zhang, D. Zhu, Y. Hu, Z. Zhou, X. Yan, H. Tian, X. Ran, Z. Luo, J. Xian, L. Yan, F. Li, L. Zeng, Y. Chen, L. Yang, S. Yan, J. Liu, M. Li, Z. Fu, H. Cheng, Lancet 371, 1753–1760 (2008)

    Article  Google Scholar 

  • N. Wilke, A. Mulcahy, S.-R. Ye, A. Morrissey, Microelectron. J. 36, 650–656 (2005)

    Article  Google Scholar 

  • Y. Wu, Y. Qiu, S. Zhang, G. Qin, Y. Gao, Biomed. Microdevices 10, 601–610 (2008)

    Article  Google Scholar 

  • B. Xu, Y. Gao, Chinese patent, CN 200310122500.4 (2003)

  • L. R. Zeitzoff, JNP, 201–206 (2005)

Download references

Acknowledgments

The work was supported by Suzhou Natong Bionanotechnology Co. Ltd, Jiangsu, China.

Author information

Authors and Affiliations

  1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    Yan Wu, Yunhua Gao, Guangjiong Qin, Suohui Zhang, Yuqin Qiu, Fang Li & Bai Xu

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Yan Wu, Guangjiong Qin & Suohui Zhang

  3. Suzhou Natong Bionanotechnology Co. Ltd, Jiangsu, 215123, China

    Bai Xu

Authors
  1. Yan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yunhua Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guangjiong Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suohui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuqin Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Bai Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yunhua Gao or Bai Xu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, Y., Gao, Y., Qin, G. et al. Sustained release of insulin through skin by intradermal microdelivery system. Biomed Microdevices 12, 665–671 (2010). https://doi.org/10.1007/s10544-010-9419-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10544-010-9419-0

Keywords

Search

Navigation