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Nitric oxide donor SNP regulates the ascorbate and glutathione metabolism in Agropyron cristatum leaves through MEK1/2

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Biologia Plantarum

Abstract

This study investigated the role of the protein kinase MEK1/2 in the regulation of ascorbate and glutathione metabolism by nitric oxide in Agropyron cristatum leaves. The results showed that NO donor, sodium nitroprusside (SNP), induced the increased activities of ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase, L-galactono-1,4-lactone dehydrogenase, and γ-glutamylcysteine synthetase (γ-ECS), the transcription of respective genes, and the content of reduced ascorbate, reduced glutathione, total ascorbate, and total glutathione. All the above increases, except the activity of γ-ECS, were suppressed by pre-treatment with MEK1/2 inhibitors 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one and 1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto) butadiene. The results of Western blot showed that SNP induced the increase in the phosphorylation of MEK1/2. Our results suggested that SNP induced the phosphorylation of MEK1/2, which, in turn, up-regulated the ascorbate and glutathione metabolism in A. cristatum leaves.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

reduced ascorbate

DHAR:

dehydroascorbate reductase

γ-ECS:

γ-glutamylcysteine synthetase

GalLDH:

L-galactono-1,4-lactone dehydrogenase

GR:

glutathione reductase

GSH:

reduced glutathione

GSSG:

oxidized glutathione

MAPK:

mitogen-activated protein kinase

MDHAR:

monodehydroascorbate reductase

PD98059:

2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

SNP:

sodium nitroprusside

U0126:

1,4-diamino-2,3-dicyano- 1,4-bis(o-amino-phenylmercapto) butadiene

References

  • Ai, L., Li, Z.H., Xie, Z.X., Tian, X.L., Eneji, A.E., Duan, L.S.: Coronatine alleviates polyethylene glycol-induced water stress in two rice (Oryza sativa L.) cultivars. — J. Agron. Crop. Sci. 194: 360–368, 2008.

    Article  CAS  Google Scholar 

  • Alemayehu, A., Zelinová, V., Bocová, B., Huttová, J., Mistrík, I., Tamás, L.: Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley. — Plant Soil 390: 213–222, 2015.

    Article  CAS  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.

    Article  CAS  PubMed  Google Scholar 

  • Dai, H., Jia, G., Shan, C.: Jasmonic acid-induced hydrogen peroxide activates MEK1/2 in upregulating the redox states of ascorbate and glutathione in wheat leaves. — Acta Physiol. Plant. 37: 200, 2015.

    Article  Google Scholar 

  • Fan, H., Du, C., Ding, L., Xu, Y.: Effects of nitric oxide on the germination of cucumber seeds and antioxidant enzymes under salinity stress. — Acta Physiol. Plant. 35: 2707–2719, 2013.

    Article  CAS  Google Scholar 

  • Favata, M.F., Horiuchi, K.Y., Manos, E.J., Daulerio, A.J., Stradley, D.A., Feeser, W.S., Van Dyk, D.E., Pitts, W.J., Earl, R.A., Hobbs, F., Copeland, R.A., Magolda, R.L., Scherle, P.A., Trzaskos, J.M.: Identification of a novel inhibitor of mitogen-activated protein kinase kinase. — J. biol. Chem. 273: 18623–18632, 1998.

    Article  CAS  PubMed  Google Scholar 

  • Griffith, O.W.: Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. — Anal. Biochem. 106: 207–212, 1980.

    Article  CAS  PubMed  Google Scholar 

  • Hasanuzzaman, M., Hossain, M.A., Fujita, M.: Nitric oxide modulates antioxidant defense and the methylglyoxal detoxification system and reduces salinity-induced damage of wheat seedlings. — Plant Biotechnol. Rep. 5: 353–365, 2011.

    Article  Google Scholar 

  • Hermes, V.S., Dall’Asta, P., Amaral, F.P., Anacleto, K.B., Arisi, A.C.M.: The regulation of transcription of genes related to oxidative stress and glutathione synthesis in Zea mays leaves by nitric oxide. — Biol. Plant. 57: 620–626, 2013.

    Article  CAS  Google Scholar 

  • Hodges, D.M., Andrews, C.J., Johnson, D.A., Hamilton, R.I.: Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines. — Plant Physiol. 98: 685–692, 1996.

    Article  CAS  Google Scholar 

  • Höller, S., Ueda, O., Wu, L., Wang, Y., Hajirezaei, M.R., Ghaffari, M.R., Wirén, N., Frei, M.: Ascorbate biosynthesis and its involvement in stress tolerance and plant development in rice (Oryza sativa L.). — Plant mol. Biol. 88: 545–560, 2015.

    Article  PubMed  Google Scholar 

  • Jonak, C., Ökrész, L., Bögre, L., Hirt, H.: Complexity, cross talk and integration of plant MAP kinase signaling. — Curr. Opin. Plant Biol. 5: 415–424, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Moon, H., Lee, B., Choi, G., Shin, D., Prasad, D.T., Lee, O., Kwak, S.S., Kim, D.H., Nam, J., Bahk, J., Hong, J.C., Lee, S.Y., Cho, M.J., Lim, C.O., Yun, D.J.: NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. — Proc. nat. Acad. Sci. USA 100: 358–363, 2003.

    Article  CAS  PubMed  Google Scholar 

  • Rüegsegger, A., Brunold, C.: Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings. — Plant Physiol. 99: 428–433, 1992.

    Article  PubMed  PubMed Central  Google Scholar 

  • Shan, C., He, F., Xu, G., Han, R., Liang, Z.: Nitric oxide is involved in the regulation of ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. — Biol. Plant. 56: 187–191, 2012.

    Article  CAS  Google Scholar 

  • Shan, C., Liang, Z.: Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. — Plant Sci. 178: 130–139, 2010.

    Article  CAS  Google Scholar 

  • Shan, C., Liang, Z., Sun, Y., Hao, W., Han, R.: The protein kinase MEK1/2 participates in the regulation of ascorbate and glutathione content by jasmonic acid in Agropyron cristatum leaves. — J. Plant Physiol. 168: 514–518, 2011.

    Article  CAS  PubMed  Google Scholar 

  • Shan, C., Yang, T.: Nitric oxide acts in the regulation of ascorbate and glutathione metabolism by jasmonic acid in Agropyron cristatum leaves. — Biol. Plant. 61: 779–784, 2017.

    Article  Google Scholar 

  • Shan, C., Zhou, Y., Liu, M.: Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress. — Protoplasma 252: 1397–1405, 2015.

    Article  CAS  PubMed  Google Scholar 

  • Tian, X., He, M., Wang, Z., Zhang, J., Song, Y., He, Z., Dong, Y.: Application of nitric oxide and calcium nitrate enhances tolerance of wheat seedlings to salt stress. — Plant Growth Regul. 77: 343–356, 2015.

    Article  CAS  Google Scholar 

  • Xiong, L., Yang, Y.: Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. — Plant Cell 15: 745–759, 2003.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yildiztugay, E., Ozfidan-Konakci, C., Kucukoduk, M.: Exogenous nitric oxide (as sodium nitroprusside) ameliorates polyethylene glycol-induced osmotic stress in hydroponically grown maize roots. — J. Plant Growth Regul. 33: 683–696, 2014.

    Article  CAS  Google Scholar 

  • Zhang, A., Jiang, M., Zhang, J., Ding, H., Xu, S., Hu, X., Tan, M.: Nitric oxide induced by hydrogen peroxide mediates abscisic acid-induced activation of the mitogen-activated protein kinase cascade involved in antioxidant defense in maize leaves. — New Phytol. 175: 36–50, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Zhao, F.Y., Cai, F.X., Gao, H.J., Zhang, S.Y., Wang, K., Liu, T., Wang, X.: ABA plays essential roles in regulating root growth by interacting with auxin and MAPK signaling pathways and cell-cycle machinery in rice seedlings. — Plant Growth Regul. 75: 535–547, 2015.

    Article  CAS  Google Scholar 

  • Zhou, B., Li, N., Zhang, Z., Huang, X., Chen, H., Hu, Z., Pang, X., Liu, W., Lu, Y.: Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis. — Biol. Plant. 56: 321–329, 2012.

    Article  CAS  Google Scholar 

  • Zottini, M., Formentin, E., Scattolin, M., Carimi, F., Lo, Schiavo, F., Terzi, M.: Nitric oxide affects plant mitochondrial functionality in vivo. — FEBS Lett. 515: 75–78, 2002.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, 453003, P.R. China

    C. Shan & N. Dong

  2. Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang, 453003, P.R. China

    C. Shan & N. Dong

Authors
  1. C. Shan
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  2. N. Dong
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Correspondence to C. Shan.

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Acknowledgements: This study was financially supported by the Innovation Fund of Science and Technology of the Henan Institute of Science and Technology in 2014 and 2015.

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Shan, C., Dong, N. Nitric oxide donor SNP regulates the ascorbate and glutathione metabolism in Agropyron cristatum leaves through MEK1/2. Biol Plant 61, 774–778 (2017). https://doi.org/10.1007/s10535-017-0712-0

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  • DOI: https://doi.org/10.1007/s10535-017-0712-0

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