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Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

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Abstract

Background and Aims

The objective of this study was to analyse the role of nitric oxide (NO) in relation to reactive oxygen species (ROS) production and root growth inhibition in the early response of barley root tip to cadmium.

Methods

We used cell-permeable NO-binding dye DAF-2DA for the NO localization and cell-impermeable dye DAR-4M for the quantification of NO in barley root apex.

Results

A considerable increase in NO production was detected in the transition zone of root tips already 30 min after the immersion of barley roots into 15 μM Cd. This Cd-induced NO generation further increased during the recovery period and reached its maximum level 2 h after the short-term treatment. At this stage an enhanced Cd-induced NO generation was observed in the meristematic, transition and in the beginning of elongation zone in comparison with control roots. Inhibitor of NO synthase activity markedly reduced root growth in control seedlings and had a synergistic growth inhibitory effect with Cd. On the contrary, this Cd-induced root growth inhibition was markedly alleviated by the NO donor. Exposure of roots to Cd markedly increased the production of ROS in the proximal elongation zone of root. While NO did not affect ROS generation, inhibition of its synthesis slightly decreased ROS generation in both control and Cd-treated roots.

Conclusions

Our results revealed that enhanced NO production is a very early response of barley root to Cd stress and it is involved in the regulation of root growth mitigating Cd-induced root growth inhibition.

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Abbreviations

DAF-2DA:

4,5-Diaminofluorescein diacetate

DAR-4M:

Diaminorhodamine-4M

NO:

Nitric oxide

L-NAME:

L-Nω-nitroarginine methyl ester

PFC:

Potassium ferricyanide

ROS:

Reactive oxygen species

SNP:

Sodium nitroprusside

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Acknowledgments

This work was supported by the Grant Agency VEGA, project No. 2/0019/13. The authors also thank the anonymous reviewers for their helpful criticisms, which improved the manuscript.

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Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic

    Aster Alemayehu, Veronika Zelinová, Beáta Bočová, Jana Huttová, Igor Mistrík & Ladislav Tamás

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  1. Aster Alemayehu
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  2. Veronika Zelinová
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  3. Beáta Bočová
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  4. Jana Huttová
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  5. Igor Mistrík
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  6. Ladislav Tamás
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Correspondence to Ladislav Tamás.

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Responsible Editor: Juan Barcelo.

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Alemayehu, A., Zelinová, V., Bočová, B. et al. Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley. Plant Soil 390, 213–222 (2015). https://doi.org/10.1007/s11104-015-2397-5

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  • DOI: https://doi.org/10.1007/s11104-015-2397-5

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