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Role of foliar application of 24-epibrassinolide in response of peanut seedlings to iron deficiency

  • Original papers
  • Published:
Biologia Plantarum

Abstract

Limited information is available on the role of brassinosteroids (BRs) in response of plants to nutrient deficiency. To understand the functions of BRs in response to iron deficiency, we investigated the effect of 24-epibrassinolide (EBR) on activities of ferric-chelate reductase (FCR), H+-ATPase, Ca2+-ATPase, nitrate reductase (NR), antioxidant enzymes, Fe and other minerals content and distribution, chlorophylls, soluble protein, free proline, reactive oxygen species, and malondialdehyde in peanut (Arachis hypogea L.) plants subjected to Fe deficiency (10−5 M Fe(III)-EDTA) with foliar application of EBR (0, 10−8, 5.0×10−8, 10−7, 5.0×10−7, and10−6 M). Results show that EBR increased Fe translocation from roots to shoots and increased Fe content in cell organelles. Activities of antioxidant enzymes increased and so the ability of resistance to oxidative stress was enhanced. As result of enhancement of H+-ATPase and Ca2+-ATPase activities, the inhibition of Fe, Ca, Mg, and Zn uptake and distribution was ameliorated. Chlorophyll, soluble protein, and free proline content also increased and consequently, chlorosis induced by Fe deficiency was alleviated. The results demonstrate that EBR had a positive role in regulating peanut growth and development under Fe deficiency and an optimal concentration appeared to be 10−7 M.

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Abbreviations

ALA:

aminolevulinic acid

BRs:

brassinosteroids

Car:

carotenoids

CAT:

catalase

Chl:

chlorophyll

CK1:

control

CK2:

low Fe treatment

EBR:

24-epibrassinolide

EBR1-5:

low Fe treatment combined with foliar application of various concentrations of EBR

FCR:

ferric-chelate reductase

MDA:

malondialdehyde

NR:

nitrate reductase

O2 •− :

superoxide anion

PM:

plasma membrane

POD:

peroxidase

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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  1. College of Resources and Environment, Shandong Agricultural University, Tai’An, 271018, P.R. China

    Y. L. Song, Y. J. Dong, X. Y. Tian, J. Kong, X. Y. Bai & L. L. Xu

  2. Institute of Food and Agricultural Sciences, University of Florida, Fort Pierce, FL, 34945, USA

    Z. L. He

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Acknowledgments: The authors thank Pingping Yang (the College of Animal Science and Technology, the Shandong Agricultural University, China) for her supplying instruments and patient guidance. This research was financially supported by the Shandong Provincial Natural Science Foundation of China (ZR2013CM003) and the Shandong Province Higher Educational Science and Technology Program (J14LF08).

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Song, Y.L., Dong, Y.J., Tian, X.Y. et al. Role of foliar application of 24-epibrassinolide in response of peanut seedlings to iron deficiency. Biol Plant 60, 329–342 (2016). https://doi.org/10.1007/s10535-016-0596-4

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