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Cloning and characterization of four novel SnRK2 genes from Triticum polonicum

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

SnRK2 are plant-specific serine/threonine kinases that are involved in plant responses to abiotic stresses. In this study, four novel genes SnRK2s:TpSnRK2.11, TpSnRK2.2, TpSnRK2.5, and TpSnRK2.10 from dwarf Polish wheat (Triticum polonicum L.) were characterized and classified into three groups. TpSnRK2.5 and TpSnRK2.11 were members of group 1; TpSnRK2.2 was member of group 2; TpSnRK2.10 belonged to group 3. The expression of TpSNRK2.2 was strongly regulated by polyethylene glycol (PEG), NaCl, and cold in roots and leaves, as well as by ABA in leaves. The transcript of TpSnRK2.5 was intensely induced by all the treatments in roots and leaves. The distinct expression patterns of TpSnRK2.10 indicate that this gene was very sensitive to ABA and NaCl, less sensitive to cold and PEG. The transcript of TpSnRK2.11 was activated significantly by PEG, NaCl, and cold, but weakly by ABA. Our results indicate that these four genes were probably involved in wheat responses to different abiotic stresses in different tissues.

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Abbreviations

ABA:

abscisic acid

AMPKs:

AMP-activated protein kinases

DPW:

dwarf Polish wheat

PEG:

polyethylene glycol

RT-qPCR:

real-time quantitative polymerase chain reaction

SNF1:

sucrose non-fermenting 1

SnRK:

SNF1-related protein kinase

UTR:

untranslated region

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, P.R. China

    Y. Wang, X. Wang, M. Gu, H. Kang, X. Fan, L. Sha, H. Zhang & Y. Zhou

  2. College of Resources and Environment, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, P.R. China

    J. Zeng

  3. Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada, Harrow, N0R 1G0, Ontario, Canada

    K. Yu

Authors
  1. Y. Wang
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  2. X. Wang
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  3. M. Gu
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  4. H. Kang
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  5. J. Zeng
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  6. X. Fan
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  7. L. Sha
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  8. H. Zhang
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  9. K. Yu
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  10. Y. Zhou
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Correspondence to Y. Zhou.

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Acknowledgements: The authors thank the National Natural Science Foundation of China (Nos. 31301349, 31270243, and 31470305), the Bureau of Science and Technology, and the Bureau of Education of Sichuan Province, China. The first two authors have equal contribution to this work.

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Wang, Y., Wang, X., Gu, M. et al. Cloning and characterization of four novel SnRK2 genes from Triticum polonicum . Biol Plant 59, 211–219 (2015). https://doi.org/10.1007/s10535-015-0501-6

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  • DOI: https://doi.org/10.1007/s10535-015-0501-6

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