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Lipid peroxidation, H2O2 content, and antioxidants during acclimatization of Abrus precatorius to ex vitro conditions

  • Published:
Biologia Plantarum

Abstract

An efficient, rapid, and reproducible plant regeneration protocol was successfully developed for Abrus precatorius L. using mature nodal explants excised from a 5-year-old field grown plant. The highest shoot regeneration frequency (87 %) with maximum number of multiple shoots (15.0) and shoot length (4.8 cm) were recorded on Murashige and Skoog (MS) medium amended with 2.5 μM thidiazuron, 120 mg dm−3 polyvinylpyrrolidone, and 0.5 μM α-naphthalene acetic acid. The best treatment for maximum root (4.0) induction was half strength MS medium supplemented with 1.5 μM indole-3-butyric acid. The in vitro plantlets with well-developed shoots and roots were successfully transferred into plastic cups with Soilrite and acclimatized in a culture room under photon flux density (PFD) of 150 μmol m−2 s−1, thereafter transferred to a greenhouse with PFD of 300 μmol m−2 s−1, and finally to a field with 70 % survival rate. During the acclimatization period (0–49 d), leaf chlorophyll and carotenoid content increased whereas malondialdehyde and H2O2 content decreased probably due to increasing activities of antioxidant enzymes (catalase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase). Our work suggests that micropropagated plants developed an antioxidant enzymatic protective system to avoid oxidative stress during establishment under ex vitro environment.

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Abbreviations

APX:

ascorbate peroxidase

BA:

6-benzyladenine

Chl:

chlorophyll

CAT:

catalase

EDTA:

ethylenediaminetetraacetic acid

GR:

glutathione reductase

IBA:

indole-3-butyric acid

KN:

kinetin

KI:

potassium iodide

MS:

Murashige and Skoog medium

MDA:

malondialdehyde

NAA:

α-naphthaleneacetic acid

NBT:

nitroblue tetrazolium

PFD:

photon flux density

PVP:

polyvinylpyrrolidone

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

TDZ:

thidiazuron

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    S. Perveen & M. Anis

  2. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    M. Anis & I. M. Aref

Authors
  1. S. Perveen
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  2. M. Anis
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  3. I. M. Aref
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Correspondence to M. Anis.

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Acknowledgements: Financial support from the DST-FIST (2011–2016) and UGC-SAP (DRS-I) programmes (2009–2014), Govt of India, New Delhi, is highly appreciated. The authors are thankful to Prof. Athar Ali Khan, Department of Statistics, Aligarh Muslim University, Aligarh, for his cooperation in data analysis.

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Perveen, S., Anis, M. & Aref, I.M. Lipid peroxidation, H2O2 content, and antioxidants during acclimatization of Abrus precatorius to ex vitro conditions. Biol Plant 57, 417–424 (2013). https://doi.org/10.1007/s10535-013-0328-y

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  • DOI: https://doi.org/10.1007/s10535-013-0328-y

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