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Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips

  • Published:
Biologia Plantarum

Abstract

The present study investigated the effects of aluminum on lipid peroxidation, accumulation of reactive oxygen species and antioxidative defense systems in root tips of wheat (Triticum aestivum L.) seedlings. Exposure to 30 μM Al increased contents of malondialdehyde, H2O2, suproxide radical and Evans blue uptake in both genotypes, with increases being greater in Al-sensitive genotype Yangmai-5 than in Al-tolerant genotype Jian-864. In addition, Al treatment increased the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), glutathione reductase (GR) and glutathione peroxidase (GPX), as well as the contents of ascorbate (AsA) and glutathione (GSH) in both genotypes. The increased activities SOD and POD were greater in Yangmai-5 than in Jian-864, whereas the opposite was true for the activities of CAT, APX, MDHAR, GR and GPX and the contents of AsA and GSH. Consequently, the antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging activity and ferric reducing/antioxidant power (FRAP) was greater in Jian-864 than in Yangmai-5.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbate

CAT:

catalase

DHAR:

dehydroascorbate reductase

DPPH:

2,2-diphenyl-1-picrylhydrazyl

FRAP:

ferric reducing/antioxidant power

GPX:

glutathione peroxidase

GR:

glutathione reductase

GSH:

reduced glutathione

GSSH:

oxidized glutathione

IRE:

inhibition of root elongation

MDA:

malondialdehyde

MDHAR:

monodehydroascorbate reductase

PCD:

programmed cell death

PM:

plasma membrane

POD:

peroxidase

ROS:

reactive oxygen species

RRE:

relative root elongation

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

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Acknowledgements

This research was financially supported by the National Basic Research Program of China (the 973 Program, No. 2007CB109305), National Natural Science Foundation of China (grant Nos. 30771292, 30900920, 30270784), the Foundation for the University Ph.D.-Granting Discipline of the Ministry of Education (grant No. 20060335014) and IPNI. Great thanks are given to Prof. Caixian Tang of La Trobe University, Australia, for his critical review of the manuscript.

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Authors and Affiliations

  1. MoE Key Laboratory of Environmental Remediation and Ecosystem Health, Hangzhou, 310058, P.R. China

    F. J. Xu, C. W. Jin & X. Y. Lin

  2. Institute of Urban Environment, Chinese Academy of Sciences, Fujian, 361021, P.R. China

    G. Li

  3. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, P.R. China

    W. J. Liu, S. S. Zhang & Y. S. Zhang

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  1. F. J. Xu
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Correspondence to X. Y. Lin.

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Xu, F.J., Li, G., Jin, C.W. et al. Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips. Biol Plant 56, 89–96 (2012). https://doi.org/10.1007/s10535-012-0021-6

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  • DOI: https://doi.org/10.1007/s10535-012-0021-6

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