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CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng

  • Original Papers
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Biologia Plantarum

Abstract

CDPKs (calcium-depended protein kinases) are of great importance for the activation of defense reactions in plants. In this study, we aimed to find a connection between CDPK expression and increased salt tolerance in Panax ginseng. Treatment of P. ginseng cell cultures with W7 (CDPK protein inhibitor) showed that CDPK proteins were necessary for salt tolerance. Expression of PgCDPK1c, PgCDPK2c and PgCDPK4a was significantly increased in the cells treated with 60 mM NaCl compared to control cells, whereas expression of PgCDPK1b and PgCDPK3a was decreased. In the NaCl-treated cells, new CDPK transcripts also appeared (PgCDPK3c, PgCDPK4as). We also used rolC and rolB transformed cultures and the effects of the rol genes on CDPK expression were similar to the effects of salt stress: they caused a significant increase in the expression of PgCDPK1c, PgCDPK2c, and PgCDPK4a and decreased expression of PgCDPK3a, in addition to the appearance of the “short” CDPK transcripts.

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Abbreviations

CDPK:

calcium-dependent protein kinase

MAPK:

mitogen-activated protein kinase

SOS3:

salt overly sensitive calcium binding proteins

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Acknowledgements

This work was supported by a grant from the Russian Foundation for Basic Research, a grant from the President of Russia (MK-714.2008.4), a grant from the Far East Division of the Russian Academy of Sciences (06-III-A-06-146) and a gra from the Russian Science Support Foundation.

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  1. Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia

    K. V. Kiselev, O. V. Grishchenko & Y. N. Zhuravlev

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  1. K. V. Kiselev
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  2. O. V. Grishchenko
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  3. Y. N. Zhuravlev
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Correspondence to K. V. Kiselev.

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Kiselev, K.V., Grishchenko, O.V. & Zhuravlev, Y.N. CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng. Biol Plant 54, 621–630 (2010). https://doi.org/10.1007/s10535-010-0112-1

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  • DOI: https://doi.org/10.1007/s10535-010-0112-1

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