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Involvement of arabinogalactan proteins in the control of cell proliferation of Cucurbita pepo suspension cultures

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Biologia Plantarum

Abstract

Arabinogalactan proteins (AGPs) secreted by zucchini squash (Cucurbita pepo L.) cell cultures into the medium are implicated in cell proliferation. Conditioned medium derived from cell suspensions of squash cultivar Dundoo could enhance multiplication rate of slow-growing cell line Cx3005. To examine the role of AGPs, a precipitation assay was performed using Yariv reagent which binds selectively to AGPs. This AGP precipitation as well as proteinase application arrested cell division. However, chitinase treatment successfully increased embryogenic callus mass. A growth promotion was also obtained by arabinogalactan addition to the culture medium. Immunoblotting analysis using the MAC 207 anti-AGP monoclonal antibody showed high AGP expression in Dundoo cell cultures.

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Abbreviations

AG:

arabinogalactan

AGPs:

arabinogalactan proteins

CM:

conditioned medium

2.4-D:

2.4-dichlorophenoxyacetic acid

MES:

2-(N-morpholino)-ethanesulfonic acid

PVDF:

polyvinylidene difluoride

β-GlucY:

β-D-glucosyl phenylglycoside

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Acknowledgements

We thank Yehia Khidr for providing us squash embryogenic calli and Marco Braun for technical assistance. We are also grateful to Güenther Buchholz for critical review of the manuscript.

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Authors and Affiliations

  1. RLP-Agroscience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, D-67435, Neustadt, Germany

    A. Ben Amar, P. Cobanov & G. M. Reustle

  2. Laboratory of Plant Molecular Physiology, CBBC, B.P. 901, Hammam-Lif, 2050, Tunisia

    A. Ben Amar, A. Ghorbel & A. Mliki

Authors
  1. A. Ben Amar
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  2. P. Cobanov
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  3. A. Ghorbel
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  4. A. Mliki
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  5. G. M. Reustle
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Correspondence to A. Ben Amar.

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Ben Amar, A., Cobanov, P., Ghorbel, A. et al. Involvement of arabinogalactan proteins in the control of cell proliferation of Cucurbita pepo suspension cultures. Biol Plant 54, 321–324 (2010). https://doi.org/10.1007/s10535-010-0055-6

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  • DOI: https://doi.org/10.1007/s10535-010-0055-6

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