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Comparison of drought-induced polypeptides and ion leakage in three tomato cultivars

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  • Published:
Biologia Plantarum

Abstract

An attempt has been made to determine if drought-induced proteins could be used as a selection marker to differentiate between tolerant and sensitive cultivars. Three Indian tomato (Lycopersicon esculentum Mill.) cultivars (Pusa Ruby, Arka Vikas and Pusa Early Dwarf) were subjected to drought stress in vivo as well as in vitro and the pattern of polypeptide expression was determined using one-dimensional SDS-PAGE. In all the three cultivars, a new 29 kDa polypeptide accumulated in leaves, in response to gradual drought stress and its accumulation was fastest in Pusa Ruby. Drought stress also resulted in an increase in ion leakage from leaf discs of all the three cultivars but the rate was lower in Pusa Ruby than in other two. Therefore, it was concluded that Pusa Ruby is most tolerant to drought stress among the three tomato cultivars investigated.

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Abbreviations

ABA:

abscisic acid

BA:

N6-benzyladenine

BSA:

bovine serum albumin

DTT:

dithiothreitol

EDTA:

ethylenediaminetetra-acetic acid

IAA:

indole-3-acetic acid

LEA:

late embryogenesis abundant

MS:

Murashige and Skoog

PAGE:

polyacrylamide gel electrophoresis

SDS:

sodium dodecyl sulphate

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    R. Roy & V. Agrawal

  2. Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi, 110007, India

    S. C. Gupta

Authors
  1. R. Roy
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  2. V. Agrawal
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  3. S. C. Gupta
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Corresponding author

Correspondence to S. C. Gupta.

Additional information

Acknowledgements: We gratefully acknowledge the courtesy of Prof. Arie Altman, the collaborator in Israel, for fruitful discussions. This work was supported by the Indo-Israel project “Molecular analysis of drought tolerance for selection and in vitro propagation of tolerant genotypes of forest trees and crop plants” sanctioned to SCG by the Department of Science and Technology, Government of India, and the University Grants Commission’s NET fellowship awarded to RR.

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Roy, R., Agrawal, V. & Gupta, S.C. Comparison of drought-induced polypeptides and ion leakage in three tomato cultivars. Biol Plant 53, 685–690 (2009). https://doi.org/10.1007/s10535-009-0123-y

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  • DOI: https://doi.org/10.1007/s10535-009-0123-y

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