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Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves

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Biologia Plantarum

Abstract

The heat stress-induced dehydrin proteins (DHNs) expression and their relationship with the water relations of sugarcane (Saccharum officinarum L.) leaves were studied. Sugarcane seedlings were subjected to heat stress (day/night temperature of 40/35 °C) under relative humidity 60/65 % to avoid aerial desiccation and determinations made at 4, 12, 24, 36, 48, 60 and 72 h. The leaves showed a sharp decline in the water and osmotic potentials, and relative water content during first 12 h of heat stress, but a regain in their values in 24 h. The pressure potential (ψp) decreased initially but increased later and approached control leaves. The increase in ψp was tightly correlated to the accumulation of free proline, glycinebetaine and soluble sugars, indicating their possible involvement in the osmotic adjustment under heat stress. Immunological detection revealed the expression of three DHNs with an apparent molecular mass of 21, 23 and 27 kDa under heat stress (48 to 72 h) and their expression was independent of the changes in the water relations of leaves.

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Abbreviations

DHNs:

dehydrin proteins

FP:

free proline

GB:

glycinebetaine

RWC:

relative water content

SS:

water soluble sugars

ψp :

pressure potential

ψs :

osmotic potential

ψw :

water potential

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Authors and Affiliations

  1. Department of Botany, University of Agriculture, Faisalabad, 38040, Pakistan

    A. Wahid

  2. Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521-0124, USA

    T. J. Close

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  1. A. Wahid
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  2. T. J. Close
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Correspondence to A. Wahid.

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Wahid, A., Close, T.J. Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves. Biol Plant 51, 104–109 (2007). https://doi.org/10.1007/s10535-007-0021-0

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  • DOI: https://doi.org/10.1007/s10535-007-0021-0

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