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Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C.A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm1, Gm2, and Gm3. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.

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Abbreviations

2,4-D:

2,4 dichlorophenoxyacetic acid

CAR:

control adventitious root

CL:

chemiluminescence

IBA:

indole-3-butyric acid

MAR:

mutated adventitious root

MS:

Murashige and Skoog

NAA:

naphthaleneacetic acid

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Authors and Affiliations

  1. Radiation Research Center for Bio-technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong, Jeongeup, Jeonbuk, 580-185, Republic of Korea

    D. S. Kim, S. Y. Kim, I. Y. Jeong, J. -B. Kim, G. -J. Lee & S. -Y. Kang

  2. Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-701, Republic of Korea

    W. Kim

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  1. D. S. Kim
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  2. S. Y. Kim
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  3. I. Y. Jeong
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  4. J. -B. Kim
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  5. G. -J. Lee
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  6. S. -Y. Kang
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  7. W. Kim
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Correspondence to D. S. Kim.

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Acknowledgements: This work was conducted as a part of a project initiated by the Korea Atomic Energy Research Institute (KAERI), and was supported by the Korean Science and Engineering Foundation (KOSEF) in the Ministry of Education, Science and Technology (MEST).

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Kim, D.S., Kim, S.Y., Jeong, I.Y. et al. Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation. Biol Plant 53, 408–414 (2009). https://doi.org/10.1007/s10535-009-0079-y

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  • DOI: https://doi.org/10.1007/s10535-009-0079-y

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