Abstract
Seed protein diversity of fourteen lentil cultivars grown in Turkey was studied by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. A distance matrix was produced based on five polymorphic protein bands, scored for their absence as 0 and presence as 1. Seed protein distances among the cultivars ranged from 0.00 to 0.80. The dendrogram based on the distance matrix indicated two distinct clusters. The first cluster includes the cultivars Sultan 1, Meyveci 2001 and Kayi 91. The second cluster contains the cultivars Pul 11, Ozbek, Emre 20, Malazgirt 89, Ciftci, Seyran 96, AliDayi, Firat 87, Sazak, Erzurum 89 and YerliKirmizi.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- SDS-PAGE:
-
sodium dodecyl sulfate polyacrylamide gel electrophoresis
- RAPD:
-
randomly amplified polymorphic DNA
References
Cherdouh, A., Khelifi, D., Carrillo, J.M., Nieto-Taladriz, M.T.: The high and low molecular weight glutenin subunit polymorphism of Algerian durum wheat landraces and old cultivars.-Plant Breed. 124: 338–342, 2005.
Ferguson, J.M., Grabe, D.F.: Identification of cultivars of perennial ryegrass by SDS-PAGE of seed proteins.-Crop Sci. 26: 170–176, 1986.
Ford, R., Pang, E.C.K., Taylor, P.W.C.: Genetics of resistance to ascochyta blight (Ascochyta lentis) of lentil and the identification of closely linked RAPD markers.-Theor. appl. Genet. 98: 93–98, 1999.
Harlan, J.R.: Anatomy of gene clusters.-Amer. Natur. 85: 97–105, 1951.
Havey, M.J., Muehlbauer, F.J.: Variability for restriction fragment lengths and phylogenies in lentil.-Theor. appl. Genet. 77: 839–843, 1989.
Hussain, A., Ramirez, H., Bushuk, W., Roca, W.: Field bean (Phaseolus vulgaris L.) cultivar identification by electrophoregrams of cotyledon storage proteins.-Euphytica 35: 729–732, 1986.
Igrejas, G., Guedes-Pinto, H., Carnide, V., Branlard, G.: Seed storage protein diversity in triticale varieties commonly grown in Portugal.-Plant Breed. 118: 303–306, 1999.
Karihaloo, J.M., Kaur, M., Singh, S.: Seed protein diversity in Solanum melongena L. and its wild and weedy relatives.-Genet. Resources Crop. Evol. 49: 533–539, 2002.
Ladizindsky, G., Hymowitz, T.: Seed protein electrophoresis in taxonomic and evolutionary studies.-Theor. appl. Genet. 54: 145–151, 1979.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680–685, 1970.
Rohlf, F.J.: NTSYS-pc Numerical Taxonomy and Multivariate Analysis System Version 1.7.-Exeter Publications, New York 1992.
Sokal, R.R., Michener, C.D.: A statistical method for evaluating systematic relationships.-Univ. Kansas Sci. Bull. 38: 1409–1438, 1958.
Stegemann, H., Afify, A.E.M.R., Hussein, K.R.F.: Identification of date (Phoenix dactylifera) cultivars by protein patterns.-Phytochemistry 26: 149–153, 1987.
Stoilova, T., Cholakova, N., Markova, M.: Variation in seed protein and isoenzyme patterns in Cucurbita cultivars.-Biol. Plant. 50: 450–452, 2006.
Vladova, R., Pandeva, R., Petcolicheva, K.: Seed storage proteins in Capsicum annuum cultivars.-Biol. Plant. 43: 291–295, 2000.
Yüzbaşıoğlu, E., Özcan, S., Açık, L.: Analysis of genetic relationships among Turkish lentil cultivars and breeding lines of Lens culinaris Medik. using RAPD markers.-Genet. Resources Crop Evol. 53: 507–514, 2006.
Zavodna, M., Kraic, J., Paglia, G., Gregova, E., Morgante, M.: Differentiation between closely related lentil (Lens culinaris Medık) cultivars using DNA markers.-Seed Sci. Technol. 28: 217–219, 2000.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yüzbaşioğlu, E., Açik, L. & Özcan, S. Seed protein diversity among lentil cultivars. Biol Plant 52, 126–128 (2008). https://doi.org/10.1007/s10535-008-0025-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-008-0025-4