Abstract
Escherichia coli ferric uptake regulator (Fur) binds a [2Fe–2S] cluster, not a mononuclear iron, when the intracellular free iron content is elevated in E. coli cells. Here we report that the C-terminal domain (residues 83–148) of E. coli Fur (Fur-CTD) is sufficient to bind the [2Fe–2S] cluster in response to elevation of the intracellular free iron content in E. coli cells. Deletion of gene fur in E. coli cells increases the intracellular free iron content and promotes the [2Fe–2S] cluster binding in the Fur-CTD in the cells grown in LB medium under aerobic growth conditions. When the Fur-CTD is expressed in wild type E. coli cells grown in M9 medium supplemented with increasing concentrations of iron, the Fur-CTD also progressively binds a [2Fe–2S] cluster with a maximum occupancy of about 36%. Like the E. coli Fur-CTD, the CTD of the Haemophilus influenzae Fur can also bind a [2Fe–2S] cluster in wild type E. coli cells grown in M9 medium supplemented with increasing concentrations of iron, indicating that binding of the [2Fe–2S] cluster in the C-terminal domain is highly conserved among Fur proteins. The results suggest that the Fur-CTD can be used as a physiological probe to assess the intracellular free iron content in bacteria.
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All data generated and analyzed in the present study are included in the manuscript. Raw data is available on request.
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This work was supported by an NSF grant (MCB 2050032).
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CF and HD conceived and designed the study, performed the experiments, analyzed the date, and wrote the manuscript. HD reviewed and edited the manuscript.
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Fontenot, C.R., Ding, H. The C-terminal domain of the ferric uptake regulator (Fur) binds a [2Fe–2S] cluster to sense the intracellular free iron content in Escherichia coli. Biometals 36, 1285–1294 (2023). https://doi.org/10.1007/s10534-023-00517-6
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DOI: https://doi.org/10.1007/s10534-023-00517-6