, Volume 21, Issue 4, pp 501–511 | Cite as

Enhanced decolourization of Direct Red-80 dye by the white rot fungus Phanerochaete chrysosporium employing sequential design of experiments

  • Sukhwinder Singh
  • Kannan Pakshirajan
  • Achlesh Daverey
Original Paper


Decolourization of Direct Red 80 (DR-80) by the white rot fungus Phanerochaete chrysosporium MTCC 787 was investigated employing sequential design of experiments. Media components for growing the white rot fungus were first screened using Plackett-Burman design and then optimized using response surface methodology (RSM), which resulted in enhancement in the efficiency of dye removal by the fungus. For determining the effect of media constituents on the dye removal, both percent dye decolourization and specific dye removal due to maximum enzyme activity were chosen as the responses from the experiments, and the media constituents glucose, veratryl alcohol, KH2PO4, CaCl2 and MgSO4 were screened to be the most effective with P values less than 0.05. Central composite design (CCD) followed by RSM in the optimization study revealed the following optimum combinations of the screened media constituents: glucose, 11.9 g l−1; veratryl alcohol, 12.03 mM; KH2PO4, 23.08 g l−1; CaCl2, 2.4 g l−1; MgSO4, 10.47 g l−1. At the optimum settings of the media constituents, complete dye decolourization (100% removal efficiency) and a maximum specific dye removal due to lignin peroxidase enzyme of 0.24 mg U−1 by the white rot fungus were observed.


Direct Red-80 Phanerochaete chrysosporium Lignin peroxidase Dye decolourization Azo dyes 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Sukhwinder Singh
    • 1
  • Kannan Pakshirajan
    • 1
  • Achlesh Daverey
    • 1
  1. 1.Department of BiotechnologyIndian Institute of TechnologyGuwahatiIndia

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