Abstract
Objective
Its eps gene cluster, the antioxidant activity and monosaccharide composition of exopolysaccharides, the expression levels of related genes at different fermentations were analyzed for clarifying the exopolysaccharide biosynthesis mechanism of Lactobacillus delbrueckii subsp. bulgaricus LDB-C1.
Results
The comparison analysis of eps gene clusters indicated that the gene clusters present diversity and strain specificity. The crude exopolysaccharides from LDB-C1 exhibited a good antioxidant activity. Compared with glucose, fructose, galactose, and fructooligosaccharide, inulin significantly improved the exopolysaccharide biosynthesis. The structures of EPSs were significantly different under different carbohydrate fermentation conditions. Inulin obviously increased the expressions of most EPS biosynthesis related genes at fermentation 4 h.
Conclusion
Inulin accelerated the beginning of the exopolysaccharide production in LDB-C1, and the enzymes promoted by inulin was beneficial for the accumulation of exopolysaccharide at the whole fermentation process.
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Supporting information
Supplementary Table 1: The information of L. delbrueckii subsp. bulgaricus strains used in this study.
Supplementary Table 2: The primers used in the qPCR analysis.
Supplementary Table 3: Carbohydrate utilization of L. delbrueckii ssp. bulgaricus strains.
Supplementary Table 4: The EPS yield of L. delbrueckii ssp. bulgaricus strain LBD-C1 under mediums containing different sugars at different fermentation stages.
Supplementary Table 5: The OD and pH of L. delbrueckii ssp. bulgaricus strain LBD-C1 under mediums containing different sugars at different fermentation stages.
Supplementary Table 6: The expressions of eps gene clusters and regulation genes of L. delbrueckii ssp. bulgaricus strain LBD-C1 under mediums containing different sugars at different fermentation stages.
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Guan, Y., Cui, Y., Wang, Q. et al. Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1. Biotechnol Lett 45, 639–654 (2023). https://doi.org/10.1007/s10529-023-03365-z
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DOI: https://doi.org/10.1007/s10529-023-03365-z