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Development of an improved procedure for isolation and purification of exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483

  • Biotechnological Products and Process Engineering
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Abstract

A method was developed for the isolation and purification of exopolysaccharide (EPS) produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483 that can be adapted for industrial-scale operation. Hydrolyzed milk medium, which was ultrafiltered to remove molecular species larger than 2.5×105 Da, was found to be a suitable growth medium for the bacteria, which produced approximately 400 mg EPS/l . Optimal isolation of EPS was achieved using centrifugation, filtration and ethanol precipitation methods. Insoluble and soluble EPS fractions were obtained. The soluble fraction was purified using a series of ethanol precipitations to achieve approximately 98% (w/w) purity. This fraction consisted of galactose, glucose, rhamnose and mannose in the ratio of approximately 5:1:0.6:0.5, with traces of glucosamine.

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Acknowledgements

This study was funded by Fonterra Co-operative Ltd and the New Zealand Foundation for Research, Science and Technology through the Technology for Business Growth program. The authors would like to acknowledge the help provided by Dr Paul McJarrow for making available the use of the Dionex electrochemical detector. The authors would like to express their gratitude to Suzanne Basset, Gary Radford and Warwick Johnson for their technical assistance. We would also like to thank Professor Richard Archer, Associate Professor Ian Maddox and Dr Vaughn Crow for helpful discussions.

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  1. Institute of Food, Nutrition and Human Health, Massey University, Private Bag 11 222, Palmerston North, New Zealand

    Kelvin K. T. Goh

  2. Riddet Centre, Massey University, Private Bag 11 222, Palmerston North, New Zealand

    Derek R. Haisman & Harjinder Singh

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  1. Kelvin K. T. Goh
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  2. Derek R. Haisman
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Correspondence to Kelvin K. T. Goh.

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Goh, K.K.T., Haisman, D.R. & Singh, H. Development of an improved procedure for isolation and purification of exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483. Appl Microbiol Biotechnol 67, 202–208 (2005). https://doi.org/10.1007/s00253-004-1739-7

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  • DOI: https://doi.org/10.1007/s00253-004-1739-7

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