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Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain

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Abstract

Objectives

To reveal the potential mechanism and key determinants that contributed to the improved pectinase activity in Aspergillus niger mutant EIMU2, which was previously obtained by UV-mutagenesis from the wild-type A. niger EIM-6.

Results

Proteomic analysis for Aspergillus niger EIMU2 by two-dimensional electrophoresis demonstrated that mutant EIMU2 harbored a multiple enzyme system for the degradation of pectin, mainly constituting by main-chain-cleaving enzymes polygalacturonase, pectate lyase, pectinesterase, and some accessory enzymes rhamnogalacturonan lyase and arabinofuranosidase. Further quantitatively differential proteomic analysis revealed that the quantities of four proteins, pectinesterase, rhamnogalacturonan lyase A, DNA-directed RNA polymerase A, and a hypothetical protein in strain EIMU2 were much higher than those in EIM-6. PCR amplification, sequencing and alignment analysis of genes for the two main members of pectin-degrading enzymes, pectate lyase and polygalacturonase showed that their sequences were completely consistent in A. niger EIM-6 and mutant EIMU2.

Conclusions

The result demonstrated that the improved pectinase activity by UV-mutagenesis in A. niger EIMU2 was probably contributed to the up-regulated expression of rhamnogalacturonan lyase, or pectinesterase, which resulted in the optimization of synergy amongst different components of pectin-degrading enzymes.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Nos. 31670125; 31660544) and the Department of Science and Technology of Fujian Province (No. 2020J01173).

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Authors and Affiliations

  1. Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, No.8, Shangsan Road, Cangshan District, Fuzhou, 350108, Fujian, China

    Weiling Lin, Ruirui Lv, Wei Huang, Hafeez ul Haq, Yuanyuan Gao, Hongli Ren, Canhua Lan & Baoyu Tian

  2. Fujian Health College, Fuzhou, 350101, Fujian, China

    Weiling Lin

  3. Library, Fujian Normal University, Fuzhou, 350108, Fujian, China

    Xiaohong Xu

  4. Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China

    Wei Huang

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  1. Weiling Lin
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Correspondence to Baoyu Tian.

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Lin, W., Xu, X., Lv, R. et al. Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain. Biotechnol Lett 43, 909–918 (2021). https://doi.org/10.1007/s10529-020-03075-w

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