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Enhanced biomass and oxidative stress tolerance of Synechococcus elongatus PCC 7942 overexpressing the DHAR gene from Brassica juncea

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Abstract

Objectives

To improve the oxidative stress tolerance, biomass yield, and ascorbate/dehydroascorbate (AsA/DHA) ratio of Synechococcus elongatus PCC 7942 in the presence of H2O2, by heterologous expression of the dehydroascorbate reductase (DHAR) gene from Brassica juncea (BrDHAR).

Results

Under H2O2 stress, overexpression of BrDHAR in the transgenic strain (BrD) of S. elongatus greatly increased the AsA/DHA ratio. As part of the AsA recycling system, the oxidative stress response induced by reactive oxygen species was enhanced, and intracellular H2O2 level decreased. In addition, under H2O2 stress conditions, the BrD strain displayed increased growth rate and biomass, as well as higher chlorophyll content and deeper pigmentation than did wild-type and control strains.

Conclusion

By maintaining the AsA pool and redox homeostasis, the heterologous expression of BrDHAR increased S. elongatus tolerance to H2O2 stress, improving the biomass yield under these conditions. The results suggest that the BrD strain of S. elongatus, with its ability to attenuate the deleterious effects of ROS caused by environmental stressors, could be a promising platform for the generation of biofuels and other valuable bioproducts.

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Acknowledgements

We thank Susan S. Golden, Spencer Diamond, and Yong-Ick Kim for helpful discussions and assistance with the Materials and Methods. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A1A05011910), Korea, and the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Science, ICT and Future Planning (2015M3A6A2065698), Korea.

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Author notes

  1. Young-Saeng Kim and Il-Sup Kim contributed equally to this work.

Authors and Affiliations

  1. Research Institute for Dok-do and Ulleung-do, Kyungpook National University, Taegu, 41566, Republic of Korea

    Young-Saeng Kim

  2. BK21 and KNU Creative BioResearch Group, Department of Energy Science, School of Life Sciences, Advanced Bio-resource Research Center, Kyungpook National University, Taegu, 41566, Republic of Korea

    Il-Sup Kim & Ho-Sung Yoon

  3. Millipore Sigma Corporation, Temecula, CA, 92590, USA

    Joseph S. Boyd

  4. Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093-0116, USA

    Arnaud Taton & James W. Golden

  5. Department of Biology, College of Natural Sciences, Kyungpook National University, Taegu, 41566, Republic of Korea

    Ho-Sung Yoon

Authors
  1. Young-Saeng Kim
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  2. Il-Sup Kim
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  3. Joseph S. Boyd
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  4. Arnaud Taton
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  5. James W. Golden
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  6. Ho-Sung Yoon
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Corresponding authors

Correspondence to James W. Golden or Ho-Sung Yoon.

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Kim, YS., Kim, IS., Boyd, J.S. et al. Enhanced biomass and oxidative stress tolerance of Synechococcus elongatus PCC 7942 overexpressing the DHAR gene from Brassica juncea . Biotechnol Lett 39, 1499–1507 (2017). https://doi.org/10.1007/s10529-017-2382-6

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  • DOI: https://doi.org/10.1007/s10529-017-2382-6

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