Abstract
Objectives
To optimize nisin production in Lactococcus lactis by using different aeration and fermentation strategies.
Results
The nisin titer and specific nisin production rate reached maximum values of 11,900 IU/ml and 4110 IU/g/h, respectively, in aerobic batch fermentation with glucose as C source. These values were higher than in anaerobic batch fermentation (10,700 IU/ml and 3260 IU/g/h, respectively). The maximum specific nisin production rates appeared earlier in aerobic batch fermentation, which suggests that nisin production is stimulated by aeration. Different fermentation strategies were compared: maximum nisin production (15,400 IU/ml) was achieved with fed-batch fermentation with a variable rate of feeding under aerobic conditions.
Conclusion
Nisin production can be stimulated by aeration, which goes against the typical conditions involving strict anaerobiosis.
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This work was supported by the Jilin Province Development and Reform Commission (2011003-1).
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Jiang, L., Liu, Y., Yan, G. et al. Aeration and fermentation strategies on nisin production. Biotechnol Lett 37, 2039–2045 (2015). https://doi.org/10.1007/s10529-015-1886-1
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DOI: https://doi.org/10.1007/s10529-015-1886-1