Abstract
Pigments produced by Monascus are traditional food colorants and are widely used as dietary supplements. Since genes involving in pigment biosynthesis have not been reported, we describe the identification of a putative pigment-regulatory gene (pigR) obtained by molecular analysis of an albino strain of Monascus ruber M7. In the pigR-deleted strain (ΔpigR), neither the pigments nor pigR expression were detected by HPLC or reverse-transcription PCR, respectively, whereas the introduction of the pigR, together with a constitutive trpC promoter into ΔpigR, caused it to produce 5.4 U of red pigments/g dry mycelia, about 12-fold higher than Monascus ruber M7 (0.46 U/g dry mycelia). Thus pigR up-regulates pigment production in Monascus ruber M7.
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Acknowledgments
We thank Dr. Daohong Jiang from Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, for providing vectors pCAMBIA3300 and pSKH and KN1. This research was financially supported by the Natural Science Foundation of China (Program: 31171649/C200101).
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Xie, N., Liu, Q. & Chen, F. Deletion of pigR gene in Monascus ruber leads to loss of pigment production. Biotechnol Lett 35, 1425–1432 (2013). https://doi.org/10.1007/s10529-013-1219-1
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DOI: https://doi.org/10.1007/s10529-013-1219-1