Skip to main content
SpringerLink
Log in

A novel phospholipase D constitutively secreted by Ochrobactrum sp. ASAG-PL1 capable of enzymatic synthesis of phosphatidylserine

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Phospholipase D (PLD) plays a crucial role in the enzyme-mediated preparation of phosphatidylserine (PS). A new PLD constitutively secreted by Ochrobactrum sp. ASAG-PL1 is now reported for the first time. After purification, the MW of the enzyme was ~37 kDa by SDS-PAGE. Its activity was highest at 40 °C and pH 7.0 and more than 90 % of the initial activity was maintained after 30 days at 4 °C and pH 7.0. This enzyme may then be useful as a potential biocatalyst for PS production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Carrea G, Arrigo PD, Piergianni V, Roncaglio S, Secundo F, Servi S (1995) Purification and properties of two phospholipases D from Streptomyces sp. Biochim Biophys Acta 1255:273–279

    Article  PubMed  Google Scholar 

  • D’Arrigo P, Cerioli L, Chiappe C, Panzeri W, Tessaro D, Mele A (2012) Improvements in the enzymatic synthesis of phosphatidylserine employing ionic liquids. J Mol Catal B 84:132–135

    Article  Google Scholar 

  • Dittrich N, Ulbrich-Hofmann R (2001) Transphosphatidylation by immobilized phospholipase D in aqueous media. Biotechnol Appl Biochem 34:189–194

    Article  PubMed  CAS  Google Scholar 

  • Duan ZQ, Hu F (2012) Highly efficient synthesis of phosphatidylserine in the eco-friendly solvent γ-valerolactone. Green Chem 6:1581–1583

    Article  Google Scholar 

  • Duan ZQ, Hu F (2013) Efficient synthesis of phosphatidylserine in 2-methyltetrahydrofuran. J Biotechnol 163:45–49

    Article  PubMed  CAS  Google Scholar 

  • Hagishita T, Nishikawa M, Hatanaka T (2000) Isolation of phospholipase D producing microorganisms with high transphosphatidylation activity. Biotechnol Lett 22:1587–1590

    Article  CAS  Google Scholar 

  • Hatanaka T, Negishi T, Mori K (2004) A mutant phospholipase D with enhanced thermostability from Streptomyces sp. Biochim Biophys Acta 1696:75–82

    Article  PubMed  CAS  Google Scholar 

  • Hellhammer J, Fries E, Buss C, Engert V, Tuch A, Rutenberg D, Hellhammer D (2004) Effects of soy lecithin phosphatidic acid and phosphatidylserine complex (PAS) on the endocrine and psychological responses to mental stress. Stress 7:119–126

    Article  PubMed  CAS  Google Scholar 

  • Hirayama S, Masuda Y, Rabeker R (2006) Effect of phosphatidylserine administration on symptoms of attention-deficit/hyperactivity disorder in children. Agro Food Ind 17:16–20

    CAS  Google Scholar 

  • Hosokawa M, Shimatani T, Kanada T, Inoue Y, Takahashi K (2000) Conversion to docosahexaenoic acid-containing phosphatidylserine from squid skin lecithin by phospholipase D-mediated transphosphatidylation. J Agric Food Chem 48:4550–4554

    Article  PubMed  CAS  Google Scholar 

  • Mander P, Simkhada JR, Cho SS, Park SJ, Choi HS, Lee HC, Sohng JK, Yoo JC (2009) A novel Ca2+-dependent phospholipase D from Streptomyces tendae, possessing only hydrolytic activity. Arch Pharm Res 32:1461–1467

    Article  PubMed  CAS  Google Scholar 

  • Nakazaw Y, Uchino M, Sagane Y, Sato H, Takano K (2009) Isolation and characterization of actinomycetes strains that produce phospholipase D having high transphosphatidylation activity. Microbiol Res 164:43–48

    Article  Google Scholar 

  • Simkhada JR, Lee HJ, Jang SY, Cho SS, Park EJ, Sohng JK, Yoo JC (2009) A novel alkalo- and thermostable phospholipase D from Streptomyces olivochromogenes. Biotechnol Lett 31:429–435

    Article  PubMed  CAS  Google Scholar 

  • Ulbrich-Hofmann R, Lerchner A, Oblozinsky M, Bezakova L (2005) Phospholipase D and its application in biocatalysis. Biotechnol Lett 27:535–544

    Article  PubMed  CAS  Google Scholar 

  • Vaisman N, Kaysar N, Zaruk-Adasha Y, Pelled D, Brichon G, Zwingelstein G, Bodennec J (2008) Correlation between changes in blood fatty acid composition and visual sustained attention performance in children with inattention: effect of dietary n-3 fatty acids containing phospholipids. Am J Clin Nutr 87:1170–1180

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We express our truly thanks for the supports from National Natural Science Foundation of China (No. 21102027) and Central Public-interest Scientific Institution Basal Research Fund (No. ZX1102).

Author information

Authors and Affiliations

  1. Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Fei Hu, Huai Wang & Ri-Sheng Yao

  2. Academy of State Administration of Grain, Beijing, 100037, People’s Republic of China

    Zhang-Qun Duan

Authors
  1. Fei Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huai Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhang-Qun Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ri-Sheng Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ri-Sheng Yao.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 250 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, F., Wang, H., Duan, ZQ. et al. A novel phospholipase D constitutively secreted by Ochrobactrum sp. ASAG-PL1 capable of enzymatic synthesis of phosphatidylserine. Biotechnol Lett 35, 1317–1321 (2013). https://doi.org/10.1007/s10529-013-1207-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-013-1207-5

Keywords

Search

Navigation