Abstract
Pre-treatment is important step prior to enzymatic hydrolysis of ligno-cellulosic biomass in order to obtain renewable carbon source ca. glucose. Pinus radiata biomass including wood blocks, wood chips and steam exploded wood (SEW) were used to investigate the effect of fungal pre-treatment on glucose yield. Comparison was made using one white-rot fungus (Trametes versicolor) and three brown-rot fungi (Coniophora puteana, Antrodia xantha and Oligoporus placenta). This is the first study where SEW was treated with basidiomycetes and subsequent enzymatic hydrolysis gave 5 g glucose/l which is an order of magnitude greater compared to control biomass (0.5 g glucose/l). This enhanced glucose yield is due to the novel pre-treatment sequence used in this study.
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References
Blanchette RA, Nilsson T, Daniel GF, Abad AR (1990) Biological degradation of wood. In: Rowell RM, Barbour J (eds) Archaeological Wood: properties. Chemistry and preservation American Chemical Society, Washington DC, USA, pp 147–174
Eaton RA, Hale MDC (1993) Wood: decay, pests, and protection. Chapman & Hall, London
Fissore A, Carrasco L, Reyes P, Rodríguez J, Freer J, Mendonça RT (2010) Evaluation of a combined brown-rot decay–chemical delignification process as a pretreatment for bioethanol production from Pinus radiata wood chips. J Ind Microbiol Biotechnol 37:893–900
Gupta R, Mehta G, Khasa YP, Kuhad RC (2010) Fungal delignification of lignocellulosic biomass improves the saccharification of cellulosics. Biodegradation. doi:10.1007/s10532-010-9404-6
Hames B, Ruiz R, Scarlata C, Sluiter A, Sluiter J, Templeton D (2008) Preparation of samples for compositional analysis LAP. National Renewable Energy Laboratory, Golden. NREL/TP-510-42620
Jovanovic I, Magnuson JK, Collart F, Robbertse B, Adney WS, Himmel ME, Baker SE (2009) Fungal glycoside hydrolases for saccharification of lignocellulose: outlook for new discoveries fuelled by genomics and functional studies. Cellulose 16:687–697
Keller FA, Hamilton JE, Nguyen QA (2003) Microbial pretreatment of biomass potential for reducing severity of thermochemical biomass pretreatment. Appl Biochem Biotechnol 105–108:27–41
Ray M, Leak D, Spanu PD, Murphy R (2010) Brown-rot fungal early stage decay mechanism as a biological pretreatment for soft wood biomass in biofuel production. Biomass Bioenergy 34:1257–1262
Sawada T, Nakamura Y, Kobayashi F, Kuwahara M, Watanabe T (1995) Effects of fungal pretreatment and steam explosion pretreatment on enzymatic saccharification of plant biomass. Biotechnol Bioeng 48:719–724
Schilling J, Tewalt J (2010) Assessment of saccharification efficacy in the cellulase system of the brown-rot fungus Gloeophyllum trabeum. Appl Microbiol Biotechnol 86:1785–1793
Schilling JS, Tewalt JP, Duncan SM (2009) Synergy between pretreatment lignocellulose modifications and saccharification efficiency in two brown-rot fungal systems. Appl Microbiol Biotechnol 84:465–475
Shi J, Sharma-Shivappa RR, Chinn M, Howell N (2009) Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioenergy 33:88–96
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Determination of Sugars, byproducts, and degradation Products in liquid fraction process samples. LAP. National Renewable Energy Laboratory, Golden; NREL/TP-510-42623
Taniguchi M, Takahashi D, Watanabe D, Sakai K, Hoshino K, Kouya T, Tanaka T (2010) Effect of steam explosion pretreatment on treatment with Pleurotus ostreatus for the enzymatic hydrolysis of rice straw. J Biosci Bioeng 110:449–452
Wong KKY, Deverell KF, Mackie KL, Clark TA, Donaldson LA (1988) The relationship between fibre porosity and cellulose digestibility in steam-exploded Pinus radiata. Biotechnol Bioeng 31:447–456
Yu H, Zhang X, Song L, Ke J, Xu C, Du W, Zhang J (2010) Evaluation of white-rot fungi-assisted alkaline/oxidative pretreatment of corn straw undergoing enzymatic hydrolysis by cellulase. J Biosci Bioeng 110:660–664
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We would like to acknowledge the help rendered by Sylke Campion and Sara Carey in the enzymatic work and compositional analysis work by Katrina Martin.
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Vaidya, A., Singh, T. Pre-treatment of Pinus radiata substrates by basidiomycetes fungi to enhance enzymatic hydrolysis. Biotechnol Lett 34, 1263–1267 (2012). https://doi.org/10.1007/s10529-012-0894-7
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DOI: https://doi.org/10.1007/s10529-012-0894-7